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Yorodumi- PDB-1u3k: The solution structure of a substrate of archaeal pre-tRNA splici... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1u3k | ||||||
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Title | The solution structure of a substrate of archaeal pre-tRNA splicing endonucleases | ||||||
Components | ABISS7 | ||||||
Keywords | RNA / bulge-helix-bulge | ||||||
Function / homology | RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / Torsion angle dynamics, molecular dynamics | ||||||
Model type details | minimized average | ||||||
Authors | Pollock, E.C. / Moore, P.B. | ||||||
Citation | Journal: To be Published Title: The solution structure of a substrate of archaeal pre-tRNA splicing endonucleases Authors: Pollock, E.C. / Moore, P.B. #1: Journal: Mol.Cell / Year: 1998 Title: Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: the bulge-helix-bulge motif Authors: Diener, J.L. / Moore, P.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1u3k.cif.gz | 248.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1u3k.ent.gz | 206.8 KB | Display | PDB format |
PDBx/mmJSON format | 1u3k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u3/1u3k ftp://data.pdbj.org/pub/pdb/validation_reports/u3/1u3k | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 12317.423 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was prepared by in vitro transcription with T7 RNA polymerase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined with residual dipolar coupling data |
-Sample preparation
Details | Contents: RNA molecule, 1-2mM in 80mM NaCl, 5mM phospate buffer pH 7, D2O Solvent system: D2O |
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Sample conditions | Ionic strength: 95mM / pH: 7 / Pressure: 1 atm / Temperature: 303 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: Torsion angle dynamics, molecular dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry, structures with the least restraint violations Conformers calculated total number: 65 / Conformers submitted total number: 10 |