Text: This structure was determined using standard 2D homonuclear and 3D heteronuclear techniques.
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試料調製
詳細
Solution-ID
内容
溶媒系
1
1 mM U-15N,13C GatB, 100 mM phosphate buffer, 75 mM KCl, 75 mM NaCl, 1 mM DTT, 1 mM sodium azide, 90% H2O, 10 % D2O
90% H2O/10% D2O
2
1 mM U-15N,13C GatB, 100 mM phosphate buffer, 75 mM KCl, 75 mM NaCl, 1 mM DTT, 1 mM sodium azide, 100 % D2O
100% D2O
3
1 mM U-15N,13C GatB, 100 mM phosphate buffer, 75 mM KCl, 75 mM NaCl, 1 mM DTT, 1 mM sodium azide, 5 mg/ml of Pf1 phage, 90% H2O, 10 % D2O
90% H2O/10% D2O
試料状態
イオン強度: 100 mM Phosphate, 75 mM NaCl, 75 mM KCl / pH: 6.8 / 圧: ambient / 温度: 298 K
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 600 MHz
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解析
NMR software
名称
バージョン
開発者
分類
XwinNMR
3.5
collection
XwinNMR
3.5
解析
NMRView
4
解析
Sparky
3.106
データ解析
TALOS
2003.027.13.05
BAX
精密化
CNS
1.1
Brunger
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: the structures are based on a total of 1377 restraints: 1119 NOE-derived distance constraints, 120 dihedral angle restraints, 56 distance restraints from hydrogen bonds (28 hydrogen bonds), ...詳細: the structures are based on a total of 1377 restraints: 1119 NOE-derived distance constraints, 120 dihedral angle restraints, 56 distance restraints from hydrogen bonds (28 hydrogen bonds), and 82 15N-1H residual dipolar couplings.
代表構造
選択基準: closest to the average
NMRアンサンブル
コンフォーマー選択の基準: structures with the least restraint violations, structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20