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- PDB-1tf9: Streptomyces griseus aminopeptidase complexed with P-Iodo-L-Pheny... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1tf9 | ||||||
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Title | Streptomyces griseus aminopeptidase complexed with P-Iodo-L-Phenylalanine | ||||||
![]() | Aminopeptidase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Reiland, V. / Gilboa, R. / Spungin-Bialik, A. / Schomburg, D. / Shoham, Y. / Blumberg, S. / Shoham, G. | ||||||
![]() | ![]() Title: Binding of inhibitory aromatic amino acids to Streptomyces griseus aminopeptidase. Authors: Reiland, V. / Gilboa, R. / Spungin-Bialik, A. / Schomburg, D. / Shoham, Y. / Blumberg, S. / Shoham, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 135 KB | Display | ![]() |
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PDB format | ![]() | 110.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | ![]() Mass: 29750.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: THE ENZYME IS ISOLATED FROM THE COMMERCIALLY AVAILABLE ENZYME MIXTURE "PRONASE E" Source: (natural) ![]() ![]() References: UniProt: P80561, ![]() | ||||||
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#2: Chemical | #3: Chemical | ChemComp-CA / | #4: Chemical | ChemComp-PHI / | #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 44.76 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: PEG 4000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 95 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Sep 27, 2001 | |||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.3→20 Å / Num. all: 63168 / Num. obs: 63168 / % possible obs: 92.7 % / Observed criterion σ(I): 0 / Redundancy: 11 % / Rsym value: 0.045 / Net I/σ(I): 17.2 | |||||||||
Reflection shell | Resolution: 1.3→1.32 Å / Redundancy: 11 % / Rsym value: 0.162 / % possible all: 57.8 |
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Processing
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Refinement | Method to determine structure![]() Resolution: 1.3→20 Å / Num. parameters: 23452 / Num. restraintsaints: 28850 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Refine analyze | Num. disordered residues: 23 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 2505.59 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.3→20 Å
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Refine LS restraints |
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