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Open data
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Basic information
Entry | Database: PDB / ID: 1t7w | |||||||||
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Title | Zn-alpha-2-glycoprotein; CHO-ZAG PEG 400 | |||||||||
![]() | Zinc-alpha-2-glycoprotein | |||||||||
![]() | LIPID BINDING PROTEIN / MHC class I homolog | |||||||||
Function / homology | ![]() Miscellaneous transport and binding events / detection of chemical stimulus involved in sensory perception of bitter taste / protein transmembrane transporter activity / RNA nuclease activity / collagen-containing extracellular matrix / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Delker, S.L. / West Jr., A.P. / McDermott, L. / Kennedy, M.W. / Bjorkman, P.J. | |||||||||
![]() | ![]() Title: Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein. Authors: Delker, S.L. / West Jr., A.P. / McDermott, L. / Kennedy, M.W. / Bjorkman, P.J. #1: ![]() Title: Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules Authors: Sanchez, L.M. / Chirino, A.J. / Bjorkman, P.J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.8 KB | Display | ![]() |
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PDB format | ![]() | 50.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1t7vSC ![]() 1t7xC ![]() 1t7yC ![]() 1t7zC ![]() 1t80C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32185.953 Da / Num. of mol.: 1 / Mutation: N89K, N92T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose![]() Source method: isolated from a genetically manipulated source |
#3: Sugar | ChemComp-NAG / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.76 Å3/Da / Density % sol: 67.27 % |
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Crystal grow![]() | Temperature: 298 K / Method: microbatch / pH: 7.5 Details: Ammonium sulfate, PEG 400, HEPES, pH 7.5, Microbatch, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 19, 2002 |
Radiation | Monochromator: Double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.7→30 Å / Num. obs: 13782 / % possible obs: 98.5 % / Redundancy: 3.2 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 12.1 |
Reflection shell | Resolution: 2.7→2.8 Å / % possible obs: 96.9 % / Rmerge(I) obs: 0.456 / Mean I/σ(I) obs: 1.8 / % possible all: 95.6 |
-Phasing
Phasing![]() | Method: ![]() |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB Entry 1T7V Resolution: 2.7→20 Å / Rfactor Rfree error: 0.011 / Occupancy max: 1 / Occupancy min: 1 / Cross valid method: THROUGHOUT / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: CNS bulk solvent model used / Bsol: 30.8686 Å2 / ksol: 0.351417 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 178.93 Å2 / Biso mean: 51.21 Å2 / Biso min: 1.51 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.7→20 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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