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Yorodumi- PDB-1t1i: High Resolution Crystal Structure of Mutant W129A of Kumamolisin,... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1t1i | ||||||
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Title | High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP) | ||||||
Components | kumamolisin | ||||||
Keywords | HYDROLASE / serine-carboxyl proteinases / subtilases / catalytic mechanism / sedolisin / kumamolisin | ||||||
Function / homology | Function and homology information tripeptidyl-peptidase activity / serine-type endopeptidase activity / proteolysis / metal ion binding Similarity search - Function | ||||||
Biological species | Bacillus sp. MN-32 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.28 Å | ||||||
Authors | Comellas-Bigler, M. / Maskos, K. / Huber, R. / Oyama, H. / Oda, K. / Bode, W. | ||||||
Citation | Journal: Structure / Year: 2004 Title: 1.2 a crystal structure of the serine carboxyl proteinase pro-kumamolisin: structure of an intact pro-subtilase Authors: Comellas-Bigler, M. / Maskos, K. / Huber, R. / Oyama, H. / Oda, K. / Bode, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t1i.cif.gz | 87.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t1i.ent.gz | 65.3 KB | Display | PDB format |
PDBx/mmJSON format | 1t1i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t1/1t1i ftp://data.pdbj.org/pub/pdb/validation_reports/t1/1t1i | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36871.566 Da / Num. of mol.: 1 / Fragment: Catalytic Domain / Mutation: W129A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus sp. MN-32 (bacteria) / Gene: KSCP / Production host: Escherichia coli (E. coli) / Strain (production host): JM 109 / References: UniProt: Q8RR56 | ||
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#2: Chemical | ChemComp-CA / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 35 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: ammonium sulfate, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: MPG/DESY, HAMBURG / Beamline: BW6 / Wavelength: 1.05 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jul 1, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.05 Å / Relative weight: 1 |
Reflection | Resolution: 1.28→50 Å / Num. obs: 74340 / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.28→50 Å / σ(F): 2 / σ(I): 2
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Refinement step | Cycle: LAST / Resolution: 1.28→50 Å
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