+Open data
-Basic information
Entry | Database: PDB / ID: 1sa9 | ||||||||||||||||||
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Title | Crystal Structure of the RNA octamer GGCGAGCC | ||||||||||||||||||
Components | 5'-R(*Keywords | RNA / RNA double helix / RNA tandem G-A base pair | Function / homology | RNA | Function and homology information Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.86 Å | Authors | Jang, S.B. / Baeyens, K. / Jeong, M.S. / SantaLucia Jr, J. / Turner, D. / Holbrook, S.R. | Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2004 | Title: Structures of two RNA octamers containing tandem G.A base pairs. Authors: Jang, S.B. / Baeyens, K. / Jeong, M.S. / SantaLucia, J. / Turner, D. / Holbrook, S.R. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sa9.cif.gz | 29.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sa9.ent.gz | 21.5 KB | Display | PDB format |
PDBx/mmJSON format | 1sa9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sa/1sa9 ftp://data.pdbj.org/pub/pdb/validation_reports/sa/1sa9 | HTTPS FTP |
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-Related structure data
Related structure data | 1saqC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 2580.617 Da / Num. of mol.: 5 / Source method: obtained synthetically / Details: RNA double helix #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.91 Å3/Da / Density % sol: 68.27 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: sodium cacodylate, PEG8000, Zn(acetate)2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Apr 3, 1995 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→60.32 Å |
Reflection shell | Resolution: 2.8→2.9 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.86→60.32 Å / σ(F): 2.5 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.86→60.32 Å
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Refine LS restraints |
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