+Open data
-Basic information
Entry | Database: PDB / ID: 1rrb | ||||||
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Title | THE RAS-BINDING DOMAIN OF RAF-1 FROM RAT, NMR, 1 STRUCTURE | ||||||
Components | RAF PROTO-ONCOGENE SERINE/THREONINE-PROTEIN KINASE | ||||||
Keywords | TRANSFERASE / RAF-1 / RAS-BINDING DOMAIN / SERINE/THREONINE-PROTEIN KINASE / RIKEN Structural Genomics/Proteomics Initiative / RSGI / Structural Genomics | ||||||
Function / homology | Function and homology information GP1b-IX-V activation signalling / CD209 (DC-SIGN) signaling / Rap1 signalling / : / small GTPase binding => GO:0031267 / Stimuli-sensing channels / RAF activation / Negative feedback regulation of MAPK pathway / Negative regulation of MAPK pathway / death-inducing signaling complex assembly ...GP1b-IX-V activation signalling / CD209 (DC-SIGN) signaling / Rap1 signalling / : / small GTPase binding => GO:0031267 / Stimuli-sensing channels / RAF activation / Negative feedback regulation of MAPK pathway / Negative regulation of MAPK pathway / death-inducing signaling complex assembly / MAP2K and MAPK activation / intermediate filament cytoskeleton organization / insulin secretion involved in cellular response to glucose stimulus / mitogen-activated protein kinase kinase binding / face development / pseudopodium / intracellular glucose homeostasis / somatic stem cell population maintenance / thyroid gland development / neurotrophin TRK receptor signaling pathway / adenylate cyclase binding / MAP kinase kinase kinase activity / negative regulation of protein-containing complex assembly / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / activation of adenylate cyclase activity / response to muscle stretch / adenylate cyclase activator activity / thymus development / MAPK cascade / positive regulation of peptidyl-serine phosphorylation / heart development / cell differentiation / non-specific serine/threonine protein kinase / response to hypoxia / protein kinase activity / intracellular signal transduction / nuclear speck / positive regulation of protein phosphorylation / negative regulation of cell population proliferation / protein phosphorylation / protein serine/threonine kinase activity / protein-containing complex binding / negative regulation of apoptotic process / Golgi apparatus / enzyme binding / positive regulation of transcription by RNA polymerase II / mitochondrion / ATP binding / identical protein binding / metal ion binding / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Terada, T. / Ito, Y. / Shirouzu, M. / Tateno, M. / Hashimoto, K. / Kigawa, T. / Ebisuzaki, T. / Takio, K. / Shibata, T. / Yokoyama, S. ...Terada, T. / Ito, Y. / Shirouzu, M. / Tateno, M. / Hashimoto, K. / Kigawa, T. / Ebisuzaki, T. / Takio, K. / Shibata, T. / Yokoyama, S. / Smith, B.O. / Laue, E.D. / Cooper, J.A. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1999 Title: Nuclear magnetic resonance and molecular dynamics studies on the interactions of the Ras-binding domain of Raf-1 with wild-type and mutant Ras proteins. Authors: Terada, T. / Ito, Y. / Shirouzu, M. / Tateno, M. / Hashimoto, K. / Kigawa, T. / Ebisuzaki, T. / Takio, K. / Shibata, T. / Yokoyama, S. / Smith, B.O. / Laue, E.D. / Cooper, J.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rrb.cif.gz | 36.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rrb.ent.gz | 23.7 KB | Display | PDB format |
PDBx/mmJSON format | 1rrb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rr/1rrb ftp://data.pdbj.org/pub/pdb/validation_reports/rr/1rrb | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11960.821 Da / Num. of mol.: 1 / Fragment: RAS-BINDING DOMAIN, RESIDUES 56-131 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Cell line: BL21 / Plasmid: PET-RID / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) References: UniProt: P11345, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C/15N-LABELED RAF-1 RBD |
-Sample preparation
Details | Contents: 20 MM SODIUM PHOSPHATE BUFFER (PH 6.5) |
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Sample conditions | Ionic strength: 5 mM MGCL2 / pH: 6.5 / Pressure: 1 atm / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: SIMULATED ANNEALING REFINEMENT FOR NMR STRUCTURE DETERMINATION | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: CLOSEST TO THE AVERAGE OF 20 LEAST ENERGY STRUCTURES Conformers calculated total number: 80 / Conformers submitted total number: 1 |