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Open data
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Basic information
Entry | Database: PDB / ID: 1rno | ||||||
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Title | RIBONUCLEASE A CRYSTALLIZED FROM 80% AMMONIUM SULPHATE | ||||||
![]() | RIBONUCLEASE A![]() | ||||||
![]() | HYDROLASE (PHOSPHORIC DIESTER) | ||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Fedorov, A.A. / Josef-Mccarthy, D. / Graf, I. / Anguelova, D. / Fedorov, E.V. / Almo, S.C. | ||||||
![]() | ![]() Title: Ionic interactions in crystalline bovine pancreatic ribonuclease A. Authors: Fedorov, A.A. / Joseph-McCarthy, D. / Fedorov, E. / Sirakova, D. / Graf, I. / Almo, S.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 33.9 KB | Display | ![]() |
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PDB format | ![]() | 26 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 93 / 2: CIS PROLINE - PRO 114 |
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Components
#1: Protein | ![]() Mass: 13708.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: CRYSTALLIZED FROM 80% AMMONIUM SULPHATE / Source: (natural) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ![]() #3: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.92 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 5.5 / Details: pH 5.5 | ||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 57 % | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.9→8.4 Å / Num. obs: 12384 / % possible obs: 94.9 % / Redundancy: 2.72 % / Rmerge(I) obs: 0.041 / Net I/σ(I): 25.62 |
Reflection shell | Resolution: 1.9→1.99 Å / Redundancy: 1.6 % / Rmerge(I) obs: 4.98 / Mean I/σ(I) obs: 0.177 / % possible all: 84.1 |
Reflection | *PLUS Highest resolution: 1.9 Å / Num. obs: 12384 / % possible obs: 94.9 % / Rmerge(I) obs: 0.041 |
Reflection shell | *PLUS Highest resolution: 1.9 Å / % possible obs: 84.1 % / Redundancy: 1.6 % / Rmerge(I) obs: 0.177 / Mean I/σ(I) obs: 4.98 |
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Processing
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Refinement | Resolution: 1.9→8 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 27.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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