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Open data
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Basic information
Entry | Database: PDB / ID: 1reu | ||||||
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Title | Structure of the bone morphogenetic protein 2 mutant L51P | ||||||
![]() | bone morphogenetic protein 2![]() | ||||||
![]() | HORMONE/GROWTH FACTOR / TGF-beta fold / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | ![]() cardiac atrium formation / cardiocyte differentiation / negative regulation of calcium-independent cell-cell adhesion / cardiac jelly development / negative regulation of aldosterone biosynthetic process / embryonic heart tube anterior/posterior pattern specification / atrioventricular canal morphogenesis / negative regulation of cortisol biosynthetic process / mesenchymal cell proliferation involved in ureteric bud development / negative regulation of steroid biosynthetic process ...cardiac atrium formation / cardiocyte differentiation / negative regulation of calcium-independent cell-cell adhesion / cardiac jelly development / negative regulation of aldosterone biosynthetic process / embryonic heart tube anterior/posterior pattern specification / atrioventricular canal morphogenesis / negative regulation of cortisol biosynthetic process / mesenchymal cell proliferation involved in ureteric bud development / negative regulation of steroid biosynthetic process / ameloblast differentiation / positive regulation of extracellular matrix constituent secretion / negative regulation of cardiac muscle cell differentiation / regulation of odontogenesis of dentin-containing tooth / endodermal-mesodermal cell signaling / corticotropin hormone secreting cell differentiation / negative regulation of insulin-like growth factor receptor signaling pathway / thyroid-stimulating hormone-secreting cell differentiation / mesenchyme development / aortic valve development / telencephalon regionalization / positive regulation of phosphatase activity / positive regulation of odontogenesis / positive regulation of cartilage development / proteoglycan metabolic process / heart induction / positive regulation of peroxisome proliferator activated receptor signaling pathway / pericardium development / lung vasculature development / BMP receptor complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Keller, S. / Nickel, J. / Zhang, J.-L. / Sebald, W. / Mueller, T.D. | ||||||
![]() | ![]() Title: Molecular recognition of BMP-2 and BMP receptor IA. Authors: Keller, S. / Nickel, J. / Zhang, J.L. / Sebald, W. / Mueller, T.D. #1: ![]() Title: Crystal structure of human bone morphogenetic protein-2 at 2.7 A resolution Authors: Scheufler, C. / Sebald, W. / Huelsmeyer, M. #2: ![]() Title: Crystal structure of the BMP-2-BRIA ectodomain complex Authors: Kirsch, T. / Sebald, W. / Dreyer, M.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 34.3 KB | Display | ![]() |
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PDB format | ![]() | 22.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1rewC ![]() 3bmpS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is a dimer generated by the two fold axis (disulfide bonded homodimer): 2/3+x-y, 1/3-y, 1/3-z |
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Components
#1: Protein | ![]() Mass: 11498.064 Da / Num. of mol.: 1 / Fragment: mature part / Mutation: L51P Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ![]() #3: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.77 Å3/Da / Density % sol: 67.1 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5 Details: lithium sulfate, tert-butanol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Apr 10, 2003 / Details: Osmic ConfocalBlue |
Radiation | Monochromator: Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.65→19.76 Å / Num. all: 5624 / Num. obs: 5624 / % possible obs: 93.5 % / Observed criterion σ(I): 1.2 / Redundancy: 3.4 % / Biso Wilson estimate: 101.2 Å2 / Rmerge(I) obs: 0.06 |
Reflection shell | Resolution: 2.65→2.82 Å / Mean I/σ(I) obs: 2.4 / Rsym value: 0.293 / % possible all: 72.3 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 3BMP Resolution: 2.65→19.76 Å / Rfactor Rfree error: 0.015 / Data cutoff high absF: 1489344.52 / Data cutoff low absF: 0 / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 48.4107 Å2 / ksol: 0.310865 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 64.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.65→19.76 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.65→2.82 Å / Rfactor Rfree error: 0.057 / Total num. of bins used: 6
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Xplor file |
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