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- PDB-1qzg: Crystal structure of Pot1 (protection of telomere)- ssDNA complex -
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Open data
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Basic information
Entry | Database: PDB / ID: 1qzg | ||||||
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Title | Crystal structure of Pot1 (protection of telomere)- ssDNA complex | ||||||
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![]() | DNA BINDING Protein/DNA / protrein-DNA complex / single-stranded telomeric DNA / DNA BINDING Protein-DNA COMPLEX | ||||||
Function / homology | ![]() Removal of the Flap Intermediate from the C-strand / telomere cap complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lei, M. / Podell, E.R. / Baumann, P. / Cech, T.R. | ||||||
![]() | ![]() Title: DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA Authors: Lei, M. / Podell, E.R. / Baumann, P. / Cech, T.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92.7 KB | Display | ![]() |
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PDB format | ![]() | 68.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The second part of the biological assembly in the asymmetric unitis generated by the following operation: matrix= ( 0.14029 0.00587 -0.99009 ) ( -0.02589 -0.99962 -0.00959 ) ( -0.98977 0.02698 -0.14009 ) translation= ( 64.45021 16.55769 73.96746 ) |
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Components
#1: DNA chain | Mass: 1535.047 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Protein | Mass: 21275.020 Da / Num. of mol.: 2 / Fragment: residues 1-185 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: POT1 / Plasmid: pET11a / Species (production host): Escherichia coli / Production host: ![]() ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.68 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 4.4 Details: PEG5000MME, TRIS, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 16 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 160 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 19, 2003 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.9→50 Å / Num. all: 32577 / Num. obs: 32234 / % possible obs: 93.2 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 |
Reflection shell | Resolution: 1.9→1.97 Å / % possible all: 71.7 |
Reflection | *PLUS Num. measured all: 103307 / Rmerge(I) obs: 0.044 |
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Processing
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Refinement | Resolution: 1.9→50 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.9→50 Å
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Refine LS restraints |
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