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Yorodumi- PDB-1qfr: NMR SOLUTION STRUCTURE OF PHOSPHOCARRIER PROTEIN HPR FROM ENTEROC... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qfr | |||||||||
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Title | NMR SOLUTION STRUCTURE OF PHOSPHOCARRIER PROTEIN HPR FROM ENTEROCOCCUS FAECALIS | |||||||||
Components | PHOSPHOCARRIER PROTEIN HPR | |||||||||
Keywords | TRANSPORT PROTEIN / HISTIDINE CONTAINING PHOSPHCARRIER PROTEIN / ENTEROCOCCUS FAECALIS / PROTEIN | |||||||||
Function / homology | Function and homology information phosphoenolpyruvate-dependent sugar phosphotransferase system / cytoplasm Similarity search - Function | |||||||||
Biological species | Enterococcus faecalis (bacteria) | |||||||||
Method | SOLUTION NMR / ANGULAR SPACE SIMULATED ANNEALING | |||||||||
Authors | Maurer, T. / Doeker, R. / Goerler, A. / Hengstenberg, W. / Kalbitzer, H.R. | |||||||||
Citation | Journal: Eur.J.Biochem. / Year: 2001 Title: Three-dimensional structure of the histidine-containing phosphocarrier protein (HPr) from Enterococcus faecalis in solution. Authors: Maurer, T. / Doker, R. / Gorler, A. / Hengstenberg, W. / Kalbitzer, H.R. #1: Journal: Eur.J.Biochem. / Year: 1998 Title: Structural studies of histidine-containing phosphocarrier protein from Enterococcus faecalis. Authors: Hahmann, M. / Maurer, T. / Lorenz, M. / Hengstenberg, W. / Glaser, S. / Kalbitzer, H.R. #2: Journal: Nature / Year: 1993 Title: Active-centre torsion-angle strain revealed in 1.6 A-resolution structure of histidine-containing phosphocarrier protein. Authors: Jia, Z. / Vandonselaar, M. / Quail, J.W. / Delbaere, L.T. #3: Journal: Biochemistry / Year: 1986 Title: Streptococcal phosphoenolpyruvate-sugar phosphotransferase system: amino acid sequence and site of ATP-dependent phosphorylation of HPr. Authors: Deutscher, J. / Pevec, B. / Beyreuther, K. / Kiltz, H.H. / Hengstenberg, W. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qfr.cif.gz | 417.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qfr.ent.gz | 345 KB | Display | PDB format |
PDBx/mmJSON format | 1qfr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qf/1qfr ftp://data.pdbj.org/pub/pdb/validation_reports/qf/1qfr | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9458.729 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Enterococcus faecalis (bacteria) / Strain: S. FAECALIS 26487 / References: UniProt: P07515 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED BY HOMONUCLEAR NMR EXPERIMENT. |
-Sample preparation
Details | Contents: 10% WATER/90% D2O |
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Sample conditions | pH: 7.4 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: ANGULAR SPACE SIMULATED ANNEALING / Software ordinal: 1 Details: STANDARD ANGULAR SPACE SIMULATED ANNEALING PROTOCOLL: CALC_ALL | ||||||||||||
NMR ensemble | Conformer selection criteria: MINIMUM PENALTY FUNCTION / Conformers calculated total number: 440 / Conformers submitted total number: 16 |