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Yorodumi- PDB-1q59: Solution Structure of the BHRF1 Protein From Epstein-Barr Virus, ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1q59 | ||||||
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Title | Solution Structure of the BHRF1 Protein From Epstein-Barr Virus, a Homolog of Human Bcl-2 | ||||||
Components | Early antigen protein R | ||||||
Keywords | VIRAL PROTEIN / BHRF1 / Bcl-2 / Epstein-Barr Virus / NMR spectroscopy / Structure determination | ||||||
Function / homology | Function and homology information symbiont-mediated suppression of host apoptosis / negative regulation of release of cytochrome c from mitochondria / host cell mitochondrion / host cell membrane / membrane Similarity search - Function | ||||||
Biological species | Human herpesvirus 4 (Epstein-Barr virus) | ||||||
Method | SOLUTION NMR / BHRF1 structures were calculated using a simulated annealing protocol with the program CNX | ||||||
Model type details | minimized average | ||||||
Authors | Huang, Q. / Petros, A.M. / Virgin, H.W. / Fesik, S.W. / Olejniczak, E.T. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: Solution Structure of the BHRF1 Protein From Epstein-Barr Virus, a Homolog of Human Bcl-2 Authors: Huang, Q. / Petros, A.M. / Virgin, H.W. / Fesik, S.W. / Olejniczak, E.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1q59.cif.gz | 64.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1q59.ent.gz | 48.8 KB | Display | PDB format |
PDBx/mmJSON format | 1q59.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q5/1q59 ftp://data.pdbj.org/pub/pdb/validation_reports/q5/1q59 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 19848.254 Da / Num. of mol.: 1 / Fragment: BH1 and BH2 domains Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human herpesvirus 4 (Epstein-Barr virus) Genus: Lymphocryptovirus / Strain: GD1 / Gene: BHRF1 / Production host: Escherichia coli (E. coli) / References: UniProt: P03182 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: U-15N,13C 0.5-1.0 mM BHRF1-EBV-BCL2 , containing 20 mM Tris (pH 7.5) and 2 mM 2H-dithiothreitol Solvent system: 90% H2O/10%2H2O |
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Sample conditions | Ionic strength: 20mM / pH: 7.5 / Pressure: ambient / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software | Name: CNS / Version: 2000 / Classification: refinement |
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Refinement | Method: BHRF1 structures were calculated using a simulated annealing protocol with the program CNX Software ordinal: 1 |
NMR representative | Selection criteria: minimized average structure |
NMR ensemble | Conformers submitted total number: 1 |