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Yorodumi- PDB-1pts: CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTID... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1pts | ||||||
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Title | CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN | ||||||
Components |
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Keywords | BIOTIN-BINDING PROTEIN/PEPTIDE / GLYCOPROTEIN / BIOTIN-BINDING PROTEIN-PEPTIDE complex | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Streptomyces avidinii (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Weber, P.C. / Pantoliano, M.W. / Thompson, L.D. | ||||||
Citation | Journal: Biochemistry / Year: 1992 Title: Crystal structure and ligand-binding studies of a screened peptide complexed with streptavidin. Authors: Weber, P.C. / Pantoliano, M.W. / Thompson, L.D. #1: Journal: J.Am.Chem.Soc. / Year: 1992 Title: Crystallographic and Thermodynamic Comparison of Natural and Synthetic Ligands Bound to Streptavidin Authors: Weber, P.C. / Wendoloski, J.J. / Pantoliano, M.W. / Salemme, F.R. #2: Journal: Science / Year: 1989 Title: Structural Origins of High-Affinity Biotin Binding to Streptavidin Authors: Weber, P.C. / Ohlendorf, D.H. / Wendoloski, J.J. / Salemme, F.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pts.cif.gz | 60.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pts.ent.gz | 44.5 KB | Display | PDB format |
PDBx/mmJSON format | 1pts.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pt/1pts ftp://data.pdbj.org/pub/pdb/validation_reports/pt/1pts | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 12738.730 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces avidinii (bacteria) / References: UniProt: P22629 #2: Protein/peptide | | Mass: 830.864 Da / Num. of mol.: 1 / Source method: obtained synthetically #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.1 % |
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Crystal grow | *PLUS Method: unknown |
-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2 Å / Num. obs: 15804 / Rmerge(I) obs: 0.069 |
-Processing
Software |
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Refinement | Rfactor Rwork: 0.179 / Rfactor obs: 0.179 / Highest resolution: 2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 10 Å / Num. reflection obs: 15592 / Rfactor obs: 0.179 / Rfactor Rwork: 0.179 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 0.039 |