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Open data
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Basic information
Entry | Database: PDB / ID: 1pcv | ||||||
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Title | Crystal structure of osmotin, a plant antifungal protein | ||||||
![]() | osmotin | ||||||
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Function / homology | ![]() response to biotic stimulus / ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Min, K. / Ha, S.C. / Yun, D.-J. / Bressan, R.A. / Kim, K.K. | ||||||
![]() | ![]() Title: Crystal structure of osmotin, a plant antifungal protein Authors: Min, K. / Ha, S.C. / Hasegawa, P.M. / Bressan, R.A. / Yun, D.-J. / Kim, K.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 89.9 KB | Display | ![]() |
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PDB format | ![]() | 68.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1aunS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22183.893 Da / Num. of mol.: 2 / Fragment: residues 1-205 / Source method: isolated from a natural source / Details: salt-adapted cultured cell / Source: (natural) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.42 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: lithium sulfate, nickel chloride, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ / pH: 7.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.3→30 Å / Num. all: 14549 / Num. obs: 13744 / % possible obs: 78.8 % / Observed criterion σ(F): 0 |
Reflection | *PLUS Num. obs: 14549 / % possible obs: 83.4 % / Num. measured all: 26588 / Rmerge(I) obs: 0.074 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1AUN Resolution: 2.3→29.19 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 65626.39 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 37.7923 Å2 / ksol: 0.383611 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→29.19 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.44 Å / Rfactor Rfree error: 0.032 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 50 Å / Rfactor Rfree![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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