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Open data
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Basic information
Entry | Database: PDB / ID: 1p2r | ||||||
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Title | T4 LYSOZYME CORE REPACKING MUTANT I78V/TA | ||||||
![]() | LYSOZYME![]() | ||||||
![]() | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() viral release from host cell by cytolysis / peptidoglycan catabolic process / cell wall macromolecule catabolic process / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Mooers, B.H. / Datta, D. / Baase, W.A. / Zollars, E.S. / Mayo, S.L. / Matthews, B.W. | ||||||
![]() | ![]() Title: Repacking the Core of T4 lysozyme by automated design Authors: Mooers, B.H. / Datta, D. / Baase, W.A. / Zollars, E.S. / Mayo, S.L. / Matthews, B.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.6 KB | Display | ![]() |
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PDB format | ![]() | 35.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1p2lC ![]() 1p36C ![]() 1p37C ![]() 1p3nC ![]() 1p46C ![]() 1p64C ![]() 1p6yC ![]() 1p7sC ![]() 1pqdC ![]() 1pqiC ![]() 1pqjC ![]() 1pqkC ![]() 1pqmC ![]() 1pqoC ![]() 1l63S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 18614.336 Da / Num. of mol.: 1 / Mutation: C54T, I78V, C97A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-K / | ||||
#3: Chemical | ![]() #4: Chemical | ChemComp-HED / | #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 47.9 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.7 Details: Potassium Phosphate, Sodium phosphate, NaCl, BME, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion / Details: Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. / PH range low: 7.1 / PH range high: 6.3 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Aug 9, 2001 / Details: Yale mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.56→19.6 Å / Num. all: 26083 / Num. obs: 26083 / % possible obs: 94.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 22.28 Å2 / Rmerge(I) obs: 0.046 / Rsym value: 0.046 / Net I/σ(I): 7.9 |
Reflection shell | Resolution: 1.56→1.67 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.274 / Mean I/σ(I) obs: 2.7 / Num. unique all: 26083 / Rsym value: 0.274 / % possible all: 94.6 |
Reflection | *PLUS Highest resolution: 1.58 Å |
Reflection shell | *PLUS % possible obs: 883 % |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1L63 Resolution: 1.58→22 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: TNT Details: THE WORKING AND TEST SETS WERE NOT COMBINED DURING THE FINAL CYCLE OF REFINEMENT.
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Solvent computation | Solvent model: TNT / Bsol: 161 Å2 / ksol: 0.7 e/Å3 | |||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.58→22 Å
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Refine LS restraints |
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Refine LS restraints | *PLUS Type: t_angle_deg / Dev ideal: 2.3 |