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Yorodumi- PDB-1owb: Three Dimensional Structure Analysis Of The Variant R109L NADH Co... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1owb | ||||||
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Title | Three Dimensional Structure Analysis Of The Variant R109L NADH Complex of Type II Citrate Synthase From E. Coli | ||||||
Components | Citrate synthase | ||||||
Keywords | TRANSFERASE / Allostery / NADH / Type II Citrate Synthase / E. Coli / R109L | ||||||
Function / homology | Function and homology information citrate synthase (unknown stereospecificity) / citrate (Si)-synthase activity / NADH binding / protein hexamerization / tricarboxylic acid cycle / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Stokell, D.J. / Donald, L.J. / Maurus, R. / Nguyen, N.T. / Sadler, G. / Choudhary, K. / Hultin, P.G. / Brayer, G.D. / Duckworth, H.W. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: Probing the roles of key residues in the unique regulatory NADH binding site of type II citrate synthase of Escherichia coli. Authors: Stokell, D.J. / Donald, L.J. / Maurus, R. / Nguyen, N.T. / Sadler, G. / Choudhary, K. / Hultin, P.G. / Brayer, G.D. / Duckworth, H.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1owb.cif.gz | 188.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1owb.ent.gz | 149.8 KB | Display | PDB format |
PDBx/mmJSON format | 1owb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ow/1owb ftp://data.pdbj.org/pub/pdb/validation_reports/ow/1owb | HTTPS FTP |
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-Related structure data
Related structure data | 1owcC 1k3p C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 48080.957 Da / Num. of mol.: 2 / Mutation: R109L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: GLTA OR GLUT OR ICDB OR B0720 / Production host: Escherichia coli (E. coli) / References: UniProt: P0ABH7, citrate (Si)-synthase #2: Chemical | ChemComp-SO4 / #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.3 Å3/Da / Density % sol: 71.37 % |
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Crystal grow | Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jul 10, 2000 / Details: osmic mirrors |
Radiation | Monochromator: Osmic mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 76417 / % possible obs: 92 % / Redundancy: 4.2 % / Rmerge(I) obs: 0.083 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1K3P 1k3p Resolution: 2.2→10 Å / σ(F): 0 / σ(I): 0 /
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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