+Open data
-Basic information
Entry | Database: PDB / ID: 1ouu | ||||||
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Title | CARBONMONOXY TROUT HEMOGLOBIN I | ||||||
Components | (HEMOGLOBIN I) x 2 | ||||||
Keywords | OXYGEN TRANSPORT / HEME / RESPIRATORY PROTEIN / ERYTHROCYTE | ||||||
Function / homology | Function and homology information haptoglobin binding / hemoglobin alpha binding / haptoglobin-hemoglobin complex / organic acid binding / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / peroxidase activity / oxygen binding / blood microparticle ...haptoglobin binding / hemoglobin alpha binding / haptoglobin-hemoglobin complex / organic acid binding / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / peroxidase activity / oxygen binding / blood microparticle / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | Oncorhynchus mykiss (rainbow trout) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | ||||||
Authors | Tame, J. / Wilson, J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1996 Title: The crystal structures of trout Hb I in the deoxy and carbonmonoxy forms. Authors: Tame, J.R. / Wilson, J.C. / Weber, R.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ouu.cif.gz | 127.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ouu.ent.gz | 99.8 KB | Display | PDB format |
PDBx/mmJSON format | 1ouu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ou/1ouu ftp://data.pdbj.org/pub/pdb/validation_reports/ou/1ouu | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 15110.572 Da / Num. of mol.: 2 / Mutation: CHAIN A, C, DEL(D32,K33) Source method: isolated from a genetically manipulated source Details: CARBONMONOXY / Source: (gene. exp.) Oncorhynchus mykiss (rainbow trout) / References: UniProt: P02019 #2: Protein | Mass: 15898.425 Da / Num. of mol.: 2 / Mutation: CHAIN A, C, DEL(D32,K33) Source method: isolated from a genetically manipulated source Details: CARBONMONOXY / Source: (gene. exp.) Oncorhynchus mykiss (rainbow trout) / Tissue: BLOOD / References: UniProt: P02142 #3: Chemical | ChemComp-HEM / #4: Chemical | ChemComp-CMO / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 49 % | ||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||
Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 22054 / % possible obs: 74.1 % / Observed criterion σ(I): 0 / Redundancy: 1.4 % / Rmerge(I) obs: 0.065 |
-Processing
Software |
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Refinement | Resolution: 2.5→8 Å / σ(F): 0
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Displacement parameters | Biso mean: 29.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→8 Å
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Refine LS restraints |
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Software | *PLUS Name: 'PROLSQ (CCP4)' / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.162 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |