+Open data
-Basic information
Entry | Database: PDB / ID: 1otf | ||||||
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Title | 4-OXALOCROTONATE TAUTOMERASE-TRICLINIC CRYSTAL FORM | ||||||
Components | 4-OXALOCROTONATE TAUTOMERASE | ||||||
Keywords | ISOMERASE / TAUTOMERASE | ||||||
Function / homology | Function and homology information 2-hydroxymuconate tautomerase / toluene catabolic process / isomerase activity Similarity search - Function | ||||||
Biological species | Pseudomonas sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Subramanya, H.S. / Roper, D.I. / Dauter, Z. / Dodson, E.J. / Davies, G.J. / Wilson, K.S. / Wigley, D.B. | ||||||
Citation | Journal: Biochemistry / Year: 1996 Title: Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases. Authors: Subramanya, H.S. / Roper, D.I. / Dauter, Z. / Dodson, E.J. / Davies, G.J. / Wilson, K.S. / Wigley, D.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1otf.cif.gz | 81 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1otf.ent.gz | 62.4 KB | Display | PDB format |
PDBx/mmJSON format | 1otf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ot/1otf ftp://data.pdbj.org/pub/pdb/validation_reports/ot/1otf | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 6984.024 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas sp. (bacteria) / Strain: CF600 / Description: T7 PROMOTER / Gene: DMPL / Plasmid: PET3A / Gene (production host): DMPL / Production host: Escherichia coli (E. coli) References: UniProt: P49172, Isomerases; Intramolecular oxidoreductases; Interconverting keto- and enol-groups #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.6 % | |||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 41 % | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 Å |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 6, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 24515 / % possible obs: 93.6 % / Redundancy: 1.9 % / Rmerge(I) obs: 0.059 |
Reflection | *PLUS Highest resolution: 1.9 Å / Rmerge(I) obs: 0.059 |
-Processing
Software |
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Refinement | Resolution: 1.9→10 Å /
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Displacement parameters | Biso mean: 18.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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