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Yorodumi- PDB-1osu: STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EX... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1osu | ||||||||||||||||||
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Title | STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS | ||||||||||||||||||
Components | RNA (5'-R(*Keywords | RNA / A-RNA / DOUBLE HELIX / NICKED / 5'-UU-OVERHANG / MISMATCHED | Function / homology | RNA | Function and homology information Method | X-RAY DIFFRACTION / Resolution: 1.4 Å | Authors | Wahl, M.C. / Rao, S.T. / Sundaralingam, M. | Citation | Journal: Nat.Struct.Biol. / Year: 1996 Title: The structure of r(UUCGCG) has a 5'-UU-overhang exhibiting Hoogsteen-like trans U.U base pairs. Authors: Wahl, M.C. / Rao, S.T. / Sundaralingam, M. #1: Journal: Nat.Struct.Biol. / Year: 1995 Title: Structure of an RNA Double Helix Including Uracil-Uracil Base Pairs in an Internal Loop Authors: Baeyens, K.J. / DeBondt, H.L. / Holbrook, S.R. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1osu.cif.gz | 11.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1osu.ent.gz | 6.6 KB | Display | PDB format |
PDBx/mmJSON format | 1osu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/os/1osu ftp://data.pdbj.org/pub/pdb/validation_reports/os/1osu | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: RNA chain | Mass: 1868.149 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.49 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K | |||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.3 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ROTATING ANODE / Type: MACSCIENCE |
Detector | Type: SIEMENS / Detector: AREA DETECTOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.4→8 Å / Num. obs: 2991 / Observed criterion σ(F): 2 |
Reflection | *PLUS % possible obs: 86.4 % / Rmerge(I) obs: 0.024 |
-Processing
Software | Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.4→8 Å / σ(F): 2
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Refine Biso |
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Refinement step | Cycle: LAST / Resolution: 1.4→8 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.4 Å / Lowest resolution: 8 Å / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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