+Open data
-Basic information
Entry | Database: PDB / ID: 1oj9 | ||||||
---|---|---|---|---|---|---|---|
Title | HUMAN MONOAMINE OXIDASE B IN COMPLEX WITH 1,4-DIPHENYL-2-BUTENE | ||||||
Components | AMINE OXIDASE [FLAVIN-CONTAINING] B | ||||||
Keywords | OXIDOREDUCTASE / FAD-CONTAINING AMINE OXIDASE / MAOB | ||||||
Function / homology | Function and homology information Biogenic amines are oxidatively deaminated to aldehydes by MAOA and MAOB / monoamine oxidase activity / monoamine oxidase / phenylethylamine catabolic process / positive regulation of dopamine metabolic process / negative regulation of serotonin secretion / response to aluminum ion / response to selenium ion / aliphatic amine oxidase activity / dopamine catabolic process ...Biogenic amines are oxidatively deaminated to aldehydes by MAOA and MAOB / monoamine oxidase activity / monoamine oxidase / phenylethylamine catabolic process / positive regulation of dopamine metabolic process / negative regulation of serotonin secretion / response to aluminum ion / response to selenium ion / aliphatic amine oxidase activity / dopamine catabolic process / primary-amine oxidase / primary amine oxidase activity / mitochondrial envelope / hydrogen peroxide biosynthetic process / response to corticosterone / substantia nigra development / response to toxic substance / flavin adenine dinucleotide binding / response to ethanol / mitochondrial outer membrane / response to lipopolysaccharide / electron transfer activity / response to xenobiotic stimulus / neuronal cell body / dendrite / mitochondrion / identical protein binding Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2.3 Å | ||||||
Authors | Binda, C. / Edmondson, D.E. / Mattevi, A. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2003 Title: Insights Into the Mode of Inhibition of Human Mitochondrial Monoamine Oxidase B from High-Resolution Crystal Structures Authors: Binda, C. / Li, M. / Hubalek, F. / Restelli, N. / Edmondson, D.E. / Mattevi, A. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1oj9.cif.gz | 204.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1oj9.ent.gz | 171.8 KB | Display | PDB format |
PDBx/mmJSON format | 1oj9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oj/1oj9 ftp://data.pdbj.org/pub/pdb/validation_reports/oj/1oj9 | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.53015, -0.4937, -0.68935), Vector: |
-Components
#1: Protein | Mass: 58837.730 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: PICHIA PASTORIS (fungus) / References: UniProt: P27338, monoamine oxidase #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.85 Å3/Da / Density % sol: 57 % | ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 6.5 / Details: pH 6.50 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7.5 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.934 |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40 Å / Num. obs: 302811 / % possible obs: 90.6 % / Redundancy: 5.8 % / Rmerge(I) obs: 0.107 / Net I/σ(I): 4.8 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.202 / Mean I/σ(I) obs: 3.5 / % possible all: 66.7 |
Reflection | *PLUS Highest resolution: 2.3 Å / Num. obs: 49954 / Redundancy: 5.8 % / Rmerge(I) obs: 0.107 |
Reflection shell | *PLUS % possible obs: 66.7 % / Rmerge(I) obs: 0.202 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: OTHER / Resolution: 2.3→40 Å / SU B: 6.155 / SU ML: 0.15 / Cross valid method: THROUGHOUT / ESU R: 0.341 / ESU R Free: 0.234
| ||||||||||||||||||||
Displacement parameters | Biso mean: 43.632 Å2
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→40 Å
| ||||||||||||||||||||
Refine LS restraints | *PLUS
|