+Open data
-Basic information
Entry | Database: PDB / ID: 1ody | ||||||
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Title | HIV-1 PROTEASE COMPLEXED WITH AN INHIBITOR LP-130 | ||||||
Components | HIV-1 PROTEASE | ||||||
Keywords | ASPARTIC PROTEASE / RETROPEPSIN / RETROVIRUS / HIV / AIDS | ||||||
Function / homology | Function and homology information integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / Uncoating of the HIV Virion / 2-LTR circle formation / Early Phase of HIV Life Cycle / Vpr-mediated nuclear import of PICs / Integration of provirus ...integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / Uncoating of the HIV Virion / 2-LTR circle formation / Early Phase of HIV Life Cycle / Vpr-mediated nuclear import of PICs / Integration of provirus / APOBEC3G mediated resistance to HIV-1 infection / Binding and entry of HIV virion / viral life cycle / Assembly Of The HIV Virion / HIV-1 retropepsin / : / retroviral ribonuclease H / Budding and maturation of HIV virion / exoribonuclease H / : / exoribonuclease H activity / protein processing / host multivesicular body / RNA-directed DNA polymerase / viral genome integration into host DNA / viral penetration into host nucleus / establishment of integrated proviral latency / RNA-directed DNA polymerase activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / RNA-DNA hybrid ribonuclease activity / peptidase activity / viral nucleocapsid / DNA recombination / Hydrolases; Acting on ester bonds / DNA-directed DNA polymerase / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / symbiont-mediated suppression of host gene expression / lipid binding / host cell nucleus / host cell plasma membrane / virion membrane / structural molecule activity / DNA binding / RNA binding / zinc ion binding / membrane / identical protein binding Similarity search - Function | ||||||
Biological species | HIV-1 M:B_HXB2R (virus) | ||||||
Method | X-RAY DIFFRACTION / RIGID BODY REFINEMENT / Resolution: 2 Å | ||||||
Authors | Kervinen, J. / Lubkowski, J. / Zdanov, A. / Wlodawer, A. / Gustchina, A. | ||||||
Citation | Journal: Protein Sci. / Year: 1998 Title: Toward a universal inhibitor of retroviral proteases: comparative analysis of the interactions of LP-130 complexed with proteases from HIV-1, FIV, and EIAV. Authors: Kervinen, J. / Lubkowski, J. / Zdanov, A. / Bhatt, D. / Dunn, B.M. / Hui, K.Y. / Powell, D.J. / Kay, J. / Wlodawer, A. / Gustchina, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ody.cif.gz | 50.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ody.ent.gz | 39.8 KB | Display | PDB format |
PDBx/mmJSON format | 1ody.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/od/1ody ftp://data.pdbj.org/pub/pdb/validation_reports/od/1ody | HTTPS FTP |
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-Related structure data
Related structure data | 2fmbC 4fivC 1odxS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10703.613 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HIV-1 M:B_HXB2R (virus) / Genus: Lentivirus / Species: Human immunodeficiency virus 1Subtypes of HIV / Production host: Escherichia coli (E. coli) / Strain (production host): HXB2 References: PIR: S63716, UniProt: P04585*PLUS, HIV-1 retropepsin #2: Chemical | ChemComp-LP1 / | #3: Water | ChemComp-HOH / | Nonpolymer details | LP-130 (HET ID LP1): ACE-NAL-VAL-STA-ABU-NAL-NH2 WHERE NAL IS NAPHTHYLALANINE, STA IS STATINE, AND ...LP-130 (HET ID LP1): ACE-NAL-VAL-STA-ABU-NAL-NH2 WHERE NAL IS NAPHTHYLAL | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.62 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.2 / Details: pH 6.2 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR591 / Wavelength: 1.5418 |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: Jul 11, 1997 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 12082 / % possible obs: 95 % / Observed criterion σ(I): 1 / Redundancy: 4.7 % / Rmerge(I) obs: 0.067 |
Reflection shell | Resolution: 2→2.07 Å / Rmerge(I) obs: 0.429 / % possible all: 85.1 |
Reflection shell | *PLUS % possible obs: 85.1 % |
-Processing
Software |
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Refinement | Method to determine structure: RIGID BODY REFINEMENT Starting model: PDB ENTRY 1ODX Resolution: 2→8 Å / σ(F): 3 /
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Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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