+Open data
-Basic information
Entry | Database: PDB / ID: 1ocw | ||||||
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Title | Free conformation Ab2 of the IgE SPE-7 | ||||||
Components | (IMMUNOGLOBULIN E) x 2 | ||||||
Keywords | IMMUNE SYSTEM / ANTIBODY / ALLERGY / CONFORMATIONAL DIVERSITY / MULTISPECIFICITY | ||||||
Function / homology | Function and homology information immunoglobulin complex / adaptive immune response / immune response / extracellular space Similarity search - Function | ||||||
Biological species | MUS MUSCULUS (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | James, L.C. / Roversi, P. / Tawfik, D. | ||||||
Citation | Journal: Science / Year: 2003 Title: Antibody Multispecificity Mediated by Conformational Diversity Authors: James, L.C. / Roversi, P. / Tawfik, D. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ocw.cif.gz | 59.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ocw.ent.gz | 43.3 KB | Display | PDB format |
PDBx/mmJSON format | 1ocw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oc/1ocw ftp://data.pdbj.org/pub/pdb/validation_reports/oc/1ocw | HTTPS FTP |
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-Related structure data
Related structure data | 1oaqSC 1oarC 1oauC 1oaxC 1oayC 1oazC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 13616.237 Da / Num. of mol.: 1 / Fragment: FV REGION, RESIDUES 1-121 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MUS MUSCULUS (house mouse) / Description: EXPRESSED AS RECOMBINANT FV IN E.COLI / Production host: ESCHERICHIA COLI (E. coli) |
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#2: Antibody | Mass: 12584.981 Da / Num. of mol.: 1 / Fragment: FV REGION, RESIDUES 1-120 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MUS MUSCULUS (house mouse) / Description: EXPRESSED AS RECOMBINANT FV IN E.COLI / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P01724*PLUS |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.5 Å3/Da / Density % sol: 40 % |
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Crystal grow | pH: 5 / Details: 0.1M HEPES, PH7.5, 70% MPD, pH 5.00 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 15, 2002 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.01→28.17 Å / Num. obs: 13952 / % possible obs: 94.4 % / Observed criterion σ(I): 2 / Redundancy: 9.9 % / Rmerge(I) obs: 0.051 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1OAQ Resolution: 2→28.17 Å Details: REFINEMENT OF TWINNING FRACTION: ALPHA=0.50(05). REFINEMENT WITH IDEALISED HYDROGEN ATOMS TWINNED DATA; APPARENT SYMMETRY I422; TWINNING OPERATOR
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Refinement step | Cycle: LAST / Resolution: 2→28.17 Å
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Refinement | *PLUS Rfactor all: 0.21 / Rfactor obs: 0.252 / Rfactor Rfree: 0.21 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |