+Open data
-Basic information
Entry | Database: PDB / ID: 1oaa | ||||||
---|---|---|---|---|---|---|---|
Title | MOUSE SEPIAPTERIN REDUCTASE COMPLEXED WITH NADP AND OXALOACETATE | ||||||
Components | SEPIAPTERIN REDUCTASE | ||||||
Keywords | OXIDOREDUCTASE / SEPIAPTERIN REDUCTASE / TETRAHYDROBIOPTERIN | ||||||
Function / homology | Function and homology information pteridine metabolic process / tetrahydrobiopterin metabolic process / sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) / sepiapterin reductase activity / L-phenylalanine metabolic process / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / eNOS activation / cell morphogenesis involved in neuron differentiation / voluntary musculoskeletal movement / serotonin metabolic process ...pteridine metabolic process / tetrahydrobiopterin metabolic process / sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) / sepiapterin reductase activity / L-phenylalanine metabolic process / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / eNOS activation / cell morphogenesis involved in neuron differentiation / voluntary musculoskeletal movement / serotonin metabolic process / tetrahydrobiopterin biosynthetic process / norepinephrine metabolic process / dopamine metabolic process / regulation of multicellular organism growth / protein homodimerization activity / mitochondrion Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 1.25 Å | ||||||
Authors | Auerbach, G. / Herrmann, A. / Bacher, A. / Huber, R. | ||||||
Citation | Journal: EMBO J. / Year: 1997 Title: The 1.25 A crystal structure of sepiapterin reductase reveals its binding mode to pterins and brain neurotransmitters. Authors: Auerbach, G. / Herrmann, A. / Gutlich, M. / Fischer, M. / Jacob, U. / Bacher, A. / Huber, R. #1: Journal: Biol.Chem. / Year: 1997 Title: The Pathway from GTP to Tetrahydrobiopterin: Three-Dimensional Structures of GTP Cyclohydrolase I and 6-Pyruvoyl Tetrahydropterin Synthase Authors: Auerbach, G. / Nar, H. #2: Journal: J.Mol.Biol. / Year: 1995 Title: 6-Pyruvoyl Tetrahydropterin Synthase, an Enzyme with a Novel Type of Active Site Involving Both Zinc Binding and an Intersubunit Catalytic Triad Motif; Site-Directed Mutagenesis of the ...Title: 6-Pyruvoyl Tetrahydropterin Synthase, an Enzyme with a Novel Type of Active Site Involving Both Zinc Binding and an Intersubunit Catalytic Triad Motif; Site-Directed Mutagenesis of the Proposed Active Center, Characterization of the Metal Binding Site and Modelling of Substrate Binding Authors: Burgisser, D.M. / Thony, B. / Redweik, U. / Hess, D. / Heizmann, C.W. / Huber, R. / Nar, H. #3: Journal: Proc.Natl.Acad.Sci.USA / Year: 1995 Title: Active Site Topology and Reaction Mechanism of GTP Cyclohydrolase I Authors: Nar, H. / Huber, R. / Auerbach, G. / Fischer, M. / Hosl, C. / Ritz, H. / Bracher, A. / Meining, W. / Eberhardt, S. / Bacher, A. #4: Journal: Structure / Year: 1995 Title: Atomic Structure of GTP Cyclohydrolase I Authors: Nar, H. / Huber, R. / Meining, W. / Schmid, C. / Weinkauf, S. / Bacher, A. #5: Journal: Embo J. / Year: 1994 Title: Three-Dimensional Structure of 6-Pyruvoyl Tetrahydropterin Synthase, an Enzyme Involved in Tetrahydrobiopterin Biosynthesis Authors: Nar, H. / Huber, R. / Heizmann, C.W. / Thony, B. / Burgisser, D. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1oaa.cif.gz | 77.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1oaa.ent.gz | 55.9 KB | Display | PDB format |
PDBx/mmJSON format | 1oaa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oa/1oaa ftp://data.pdbj.org/pub/pdb/validation_reports/oa/1oaa | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-Components
#1: Protein | Mass: 27648.684 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Organ: BRAIN / Cellular location (production host): CYTOSOL / Production host: Escherichia coli (E. coli) References: UniProt: Q64105, sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) | ||||||
---|---|---|---|---|---|---|---|
#2: Chemical | #3: Chemical | ChemComp-OAA / | #4: Chemical | ChemComp-NAP / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.66 Å3/Da / Density % sol: 66 % | |||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.5 / Method: vapor diffusion | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 95 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: MPG/DESY, HAMBURG / Beamline: BW6 / Wavelength: 1 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 1, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.25→8 Å / Num. obs: 112800 / % possible obs: 99.6 % / Observed criterion σ(I): 0 / Redundancy: 2.9 % / Rmerge(I) obs: 0.068 |
Reflection shell | Resolution: 1.25→1.28 Å / Redundancy: 2.9 % / % possible all: 97.3 |
Reflection | *PLUS Num. measured all: 630228 |
Reflection shell | *PLUS % possible obs: 97.3 % |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MIR / Resolution: 1.25→6 Å / Cross valid method: FREE-R / σ(F): 0
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.25→6 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 29 Å2 |