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Open data
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Basic information
Entry | Database: PDB / ID: 1nz3 | ||||||
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Title | K45E-K63E Variant of Horse Heart Myoglobin | ||||||
![]() | Myoglobin![]() | ||||||
![]() | OXYGEN STORAGE/TRANSPORT / Horse heart myoglobin / OXYGEN STORAGE-TRANSPORT COMPLEX | ||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Hunter, C.L. / Maurus, R. / Mauk, M.R. / Lee, H. / Raven, E.L. / Tong, H. / Nguyen, N. / Smith, S. / Brayer, G.D. / Mauk, A.G. | ||||||
![]() | ![]() Title: Introduction and characterization of a functionally linked metal ion binding site at the exposed heme edge of myoglobin Authors: Hunter, C.L. / Maurus, R. / Mauk, M.R. / Lee, H. / Raven, E.L. / Tong, H. / Nguyen, N. / Smith, S. / Brayer, G.D. / Mauk, A.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.2 KB | Display | ![]() |
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PDB format | ![]() | 30.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1nz2C ![]() 1nz4C ![]() 1nz5C ![]() 1wlaS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 16983.383 Da / Num. of mol.: 1 / Mutation: K45E, K63E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-HEM / ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.92 Å3/Da / Density % sol: 36.01 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.3 Details: ammonium sulfate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 25 ℃ | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.6→10 Å / Num. obs: 14180 / % possible obs: 79 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.097 |
Reflection | *PLUS Lowest resolution: 10 Å / % possible obs: 79 % |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1WLA Resolution: 1.6→10 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber /
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Refinement step | Cycle: LAST / Resolution: 1.6→10 Å
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Refinement | *PLUS Lowest resolution: 10 Å / Rfactor Rwork![]() | ||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||
Refine LS restraints | *PLUS
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