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- PDB-1nsy: CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1nsy | ||||||
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Title | CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS | ||||||
![]() | NAD SYNTHETASE | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Rizzi, M. / Nessi, C. / Bolognesi, M. / Galizzi, A. / Coda, A. | ||||||
![]() | ![]() Title: Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis. Authors: Rizzi, M. / Nessi, C. / Mattevi, A. / Coda, A. / Bolognesi, M. / Galizzi, A. #1: ![]() Title: Crystallization of Nad+ Synthetase from Bacillus Subtilis Authors: Rizzi, M. / Nessi, C. / Bolognesi, M. / Coda, A. / Galizzi, A. #2: ![]() Title: The Outb Gene of Bacillus Subtilis Codes for Nad Synthetase Authors: Nessi, C. / Albertini, A.M. / Speranza, M.L. / Galizzi, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 125.4 KB | Display | ![]() |
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PDB format | ![]() | 97.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 30303.994 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() References: UniProt: P08164, ![]() |
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-Non-polymers , 5 types, 233 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/AMP.gif)
![](data/chem/img/POP.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/AMP.gif)
![](data/chem/img/POP.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ![]() #4: Chemical | ![]() #5: Chemical | ![]() #6: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 43 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | *PLUS Temperature: 28 ℃ / pH: 5.2 / Method: microdialysis | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Apr 5, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Num. obs: 33464 / % possible obs: 94 % / Redundancy: 5.2 % / Rmerge(I) obs: 0.064 |
Reflection | *PLUS Highest resolution: 2 Å / Num. measured all: 177041 |
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Processing
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Refinement | Resolution: 2→15 Å / Rfactor Rfree![]() ![]() | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→15 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.174 | ||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |