+
Open data
-
Basic information
Entry | Database: PDB / ID: 1nj9 | ||||||
---|---|---|---|---|---|---|---|
Title | Cocaine hydrolytic antibody 15A10 | ||||||
![]() |
| ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Larsen, N.A. / de Prada, P. / Deng, S.X. / Zhu, X. / Landry, D.W. / Wilson, I.A. | ||||||
![]() | ![]() Title: Crystallographic and biochemical analysis of cocaine-degrading antibody 15A10. Authors: Larsen, N.A. / de Prada, P. / Deng, S.X. / Mittal, A. / Braskett, M. / Zhu, X. / Wilson, I.A. / Landry, D.W. #1: ![]() Title: Structure determination of a cocaine hydrolytic antibody from a pseudomerohedrally twinned crystal. Authors: Larsen, N.A. / Heine, A. / de Prada, P. / Redwan, E.R. / Yeates, T.O. / Landry, D.W. / Wilson, I.A. | ||||||
History |
| ||||||
Remark 999 | Sequence an appropriate sequence database reference was not available at the time of processing. |
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 169.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 138.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Antibody | Mass: 22980.430 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() #2: Antibody | ![]() Mass: 23015.744 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() #3: Chemical | ChemComp-NA / #4: Water | ChemComp-HOH / | ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.98 % | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow![]() | Temperature: 296 K / Method: vapor diffusion, sitting drop / pH: 5.4 Details: 12% PEG 4000, 12-14% isopropanol, 100mM MES, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 296K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 296 K / pH: 5.5 / Method: vapor diffusion, sitting dropDetails: Larsen, N.A., (2002) Acta Crystallogr.,Sect.D, 58, 2055. | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 120 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 20, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.35→30 Å / Num. obs: 32009 / % possible obs: 85.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 2.9 % / Biso Wilson estimate: 35 Å2 / Rmerge(I) obs: 0.083 / Rsym value: 0.083 / Net I/σ(I): 14.4 |
Reflection shell | Resolution: 2.35→2.45 Å / Rmerge(I) obs: 0.399 / Mean I/σ(I) obs: 2.8 / Rsym value: 0.399 / % possible all: 76.6 |
Reflection | *PLUS Num. measured all: 91574 |
Reflection shell | *PLUS % possible obs: 76.6 % |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]()
| |||||||||||||||||||||||||
Refine analyze |
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.35→30 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
Software | *PLUS Name: SHELX / Version: 97 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rwork![]() | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
|