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Yorodumi- PDB-1n89: Solution structure of a liganded type 2 wheat non-specific Lipid ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1n89 | ||||||
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Title | Solution structure of a liganded type 2 wheat non-specific Lipid Transfer Protein | ||||||
Components | lipid transfer proteinPlant lipid transfer proteins | ||||||
Keywords | LIPID TRANSPORT / lipid transfer protein | ||||||
Function / homology | Function and homology information lipid transport / cell wall organization / lipid binding / extracellular region Similarity search - Function | ||||||
Biological species | Triticum turgidum subsp. durum (durum wheat) | ||||||
Method | SOLUTION NMR / annealing protocol | ||||||
Model type details | minimized average | ||||||
Authors | Pons, J.L. / de Lamotte, F. / Gautier, M.F. / Delsuc, M.A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: Refined solution structure of a liganded type 2 wheat nonspecific lipid transfer protein. Authors: Pons, J.L. / de Lamotte, F. / Gautier, M.F. / Delsuc, M.A. | ||||||
History |
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Remark 650 | HELIX Author determined the secondary structure. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1n89.cif.gz | 174.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1n89.ent.gz | 146 KB | Display | PDB format |
PDBx/mmJSON format | 1n89.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n8/1n89 ftp://data.pdbj.org/pub/pdb/validation_reports/n8/1n89 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6985.943 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Triticum turgidum subsp. durum (durum wheat) Species: Triticum turgidum / Strain: subsp. durum / Production host: Pichia pastoris (fungus) / Strain (production host): GS115 / References: UniProt: P82900 |
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#2: Chemical | ChemComp-PGM / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2.8 mmol 15N-LTP with 1.5 eq PGM / Solvent system: 70 mmol Phosphate |
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Sample conditions | pH: 3.5 / Pressure: ambient / Temperature: 295.2 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AMX / Manufacturer: Bruker / Model: AMX / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: annealing protocol / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 250 / Conformers submitted total number: 9 |