+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1n72 | |||||||||
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タイトル | Structure and Ligand of a Histone Acetyltransferase Bromodomain | |||||||||
要素 | HISTONE ACETYLTRANSFERASEヒストンアセチルトランスフェラーゼ | |||||||||
キーワード | TRANSFERASE (転移酵素) / Histone acetyltransferase bromodomain / 4-helical bundle | |||||||||
機能・相同性 | 機能・相同性情報 regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / diamine N-acetyltransferase activity / positive regulation of transcription from RNA polymerase II promoter by glucose / negative regulation of centriole replication / Physiological factors / positive regulation of attachment of mitotic spindle microtubules to kinetochore / peptidyl-lysine acetylation ...regulation of protein ADP-ribosylation / negative regulation of rRNA processing / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / diamine N-acetyltransferase activity / positive regulation of transcription from RNA polymerase II promoter by glucose / negative regulation of centriole replication / Physiological factors / positive regulation of attachment of mitotic spindle microtubules to kinetochore / peptidyl-lysine acetylation / lysine N-acetyltransferase activity, acting on acetyl phosphate as donor / YAP1- and WWTR1 (TAZ)-stimulated gene expression / positive regulation of fatty acid biosynthetic process / ミオフィラメント / histone H3 acetyltransferase activity / internal peptidyl-lysine acetylation / cyclin-dependent protein serine/threonine kinase inhibitor activity / N-terminal peptidyl-lysine acetylation / ATAC complex / I band / cellular response to parathyroid hormone stimulus / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / SAGA complex / RUNX3 regulates NOTCH signaling / limb development / A band / NOTCH4 Intracellular Domain Regulates Transcription / Regulation of FOXO transcriptional activity by acetylation / NOTCH3 Intracellular Domain Regulates Transcription / regulation of tubulin deacetylation / peptide-lysine-N-acetyltransferase activity / histone acetyltransferase binding / protein acetylation / Notch-HLH transcription pathway / Formation of WDR5-containing histone-modifying complexes / regulation of cell division / Formation of paraxial mesoderm / acetyltransferase activity / RNA Polymerase I Transcription Initiation / regulation of RNA splicing / regulation of embryonic development / regulation of DNA repair / histone acetyltransferase activity / positive regulation of gluconeogenesis / ヒストンアセチルトランスフェラーゼ / transcription initiation-coupled chromatin remodeling / positive regulation of glycolytic process / 糖新生 / transcription coregulator activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / B-WICH complex positively regulates rRNA expression / 紡錘体 / NOTCH1 Intracellular Domain Regulates Transcription / Metalloprotease DUBs / 動原体 / 記憶 / Pre-NOTCH Transcription and Translation / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / positive regulation of neuron projection development / histone deacetylase binding / cellular response to insulin stimulus / vasodilation / rhythmic process / cellular response to oxidative stress / heart development / HATs acetylate histones / DNA-binding transcription factor binding / Estrogen-dependent gene expression / transcription coactivator activity / regulation of cell cycle / クロマチンリモデリング / 細胞周期 / negative regulation of cell population proliferation / 中心体 / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / 核質 / 細胞核 / 細胞質基質 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 溶液NMR / distance geometry simulated annealing, molecular dynamics matrix relaxation torsion angle dynamics | |||||||||
データ登録者 | Dhalluin, C. / Carlson, J.E. / Zeng, L. / He, C. / Aggarwal, A.K. / Zhou, M.-M. | |||||||||
引用 | ジャーナル: Nature / 年: 1999 タイトル: Structure and Ligand of a Histone Acetyltransferase Bromodomain 著者: Dhalluin, C. / Carlson, J.E. / Zeng, L. / He, C. / Aggarwal, A.K. / Zhou, M.-M. | |||||||||
履歴 |
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Remark 999 | SEQUENCE The authors believe PRO is at both positions 804 and 805 in the wild protein sequence. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1n72.cif.gz | 48.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1n72.ent.gz | 34.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1n72.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n7/1n72 ftp://data.pdbj.org/pub/pdb/validation_reports/n7/1n72 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 14052.226 Da / 分子数: 1 / 断片: Bromodomain / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: PCAF / プラスミド: pET14b / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) 参照: UniProt: Q92831, ヒストンアセチルトランスフェラーゼ |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: 1mM Bromodomain, 100mM Sodium phosphate, 5mM DTT-d10, 0.5mM EDTA, pH 6.5 溶媒系: 90% H2O/10% D2O |
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試料状態 | イオン強度: 200 / pH: 6.5 / 圧: 1 atm / 温度: 303 K |
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M | |||||||||||||||
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放射波長 | 相対比: 1 | |||||||||||||||
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: distance geometry simulated annealing, molecular dynamics matrix relaxation torsion angle dynamics ソフトェア番号: 1 | |||||||||||||||
NMRアンサンブル | 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 1 |