[English] 日本語
Yorodumi- PDB-1n3j: Structure and Substrate of a Histone H3 Lysine Methyltransferase ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1n3j | ||||||
---|---|---|---|---|---|---|---|
Title | Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1 | ||||||
Components | Histone H3 Lysine Methyltransferase | ||||||
Keywords | TRANSFERASE / Beta barrel / homodimer | ||||||
Function / homology | Function and homology information histone H3K37 methyltransferase activity / methylation / identical protein binding Similarity search - Function | ||||||
Biological species | Paramecium bursaria Chlorella virus 1 | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing, molecular dynamics | ||||||
Model type details | minimized average | ||||||
Authors | Manzur, K.L. / Farooq, A. / Zeng, L. / Plotnikova, O. / Sachchidanand / Koch, A.W. / Zhou, M.-M. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2003 Title: A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3. Authors: Manzur, K.L. / Farooq, A. / Zeng, L. / Plotnikova, O. / Koch, A.W. / Sachchidanand / Zhou, M.-M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1n3j.cif.gz | 86 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1n3j.ent.gz | 65.6 KB | Display | PDB format |
PDBx/mmJSON format | 1n3j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n3/1n3j ftp://data.pdbj.org/pub/pdb/validation_reports/n3/1n3j | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 13606.623 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Paramecium bursaria Chlorella virus 1 / Genus: Chlorovirus / Gene: A612L / Plasmid: pET22b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O41094 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 0.5mM Protein, 50mM Sodium phosphate, 700mM NaCl, 300mM Urea, 0.1mM EDTA, 5mM DTT-d10 Solvent system: 90% H2O/10% D2O |
---|---|
Sample conditions | Ionic strength: 1M / pH: 6.5 / Pressure: 1 atm / Temperature: 310 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
---|---|
Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
-Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: distance geometry simulated annealing, molecular dynamics Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: mimized average structure / Conformers calculated total number: 200 / Conformers submitted total number: 1 |