+Open data
-Basic information
Entry | Database: PDB / ID: 1mwm | ||||||
---|---|---|---|---|---|---|---|
Title | ParM from plasmid R1 ADP form | ||||||
Components | ParM | ||||||
Keywords | STRUCTURAL PROTEIN / ParM | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Van den Ent, F. / Moller-Jensen, J. / Amos, L.A. / Gerdes, K. / Lowe, J. | ||||||
Citation | Journal: EMBO J. / Year: 2002 Title: F-actin-like filaments formed by plasmid segregation protein ParM Authors: Van den Ent, F. / Moller-Jensen, J. / Amos, L.A. / Gerdes, K. / Lowe, J. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1mwm.cif.gz | 144.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1mwm.ent.gz | 113.4 KB | Display | PDB format |
PDBx/mmJSON format | 1mwm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1mwm_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1mwm_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 1mwm_validation.xml.gz | 30.8 KB | Display | |
Data in CIF | 1mwm_validation.cif.gz | 43.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mw/1mwm ftp://data.pdbj.org/pub/pdb/validation_reports/mw/1mwm | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 35804.375 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: pMD137 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P11904 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.86 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 6.2 Details: 100mM Na-cacodyltae, 400mM NaCl, 400mM Mg-acetate, 11% PEG8000, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX14.2 / Wavelength: 0.96 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 20, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96 Å / Relative weight: 1 |
Reflection | Resolution: 1.99→21.9 Å / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection | *PLUS Highest resolution: 2 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.064 |
Reflection shell | *PLUS Mean I/σ(I) obs: 4.6 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Semet ParM Resolution: 2→500 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→500 Å
| ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 500 Å / Rfactor Rfree: 0.252 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
|