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Open data
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Basic information
Entry | Database: PDB / ID: 1mpr | ||||||
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Title | MALTOPORIN FROM SALMONELLA TYPHIMURIUM | ||||||
![]() | MALTOPORIN![]() | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() maltodextrin transmembrane transporter activity / maltose transporting porin activity / polysaccharide transport / ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Meyer, J.E.W. / Schulz, G.E. | ||||||
![]() | ![]() Title: Structure of maltoporin from Salmonella typhimurium ligated with a nitrophenyl-maltotrioside. Authors: Meyer, J.E. / Hofnung, M. / Schulz, G.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 260.6 KB | Display | ![]() |
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PDB format | ![]() | 213.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2mprC ![]() 1malS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Details | THE FOLLOWING TRANSFORMATION WILL TRANSFORM THE WATERS OF CHAIN A INTO THE WATERS OF CHAIN B. MTRIX1 -0.485848 -0.865005 -0.125370 65.39400 MTRIX2 0.864287 -0.496828 0.078542 99.80500 MTRIX3 -0.130227 -0.070196 0.988996 5.42100 THE FOLLOWING TRANSFORMATION WILL TRANSFORM THE WATERS OF CHAIN A INTO THE WATERS OF CHAIN C. MTRIX1 -0.488651 0.862290 -0.132950 -53.38000 MTRIX2 -0.862650 -0.500312 -0.074311 107.08700 MTRIX3 -0.130594 0.078377 0.988333 -4.78000 |
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Components
#1: Protein | ![]() Mass: 48064.535 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Chemical | ChemComp-CA / | #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.3 Å3/Da / Density % sol: 71 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Method: vapor diffusion, hanging drop Details: MALTOPORIN WAS CRYSTALLIZED BY HANGING-DROP METHOD. DROP: 5-8 MG/ML PROTEIN, 0.3% C8E4, 0.8% C6DAO, 1 MM CACL2, 1MM MGCL2, 14-18% PEG 1500, 0.02% NAN3 RESERVOIR: 28-32% PEG 1500, vapor diffusion - hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 14, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.8→20 Å / Num. obs: 59257 / % possible obs: 95 % / Redundancy: 2.9 % / Biso Wilson estimate: 45 Å2 / Rsym value: 0.072 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 2.77→2.84 Å / Redundancy: 2.4 % / Mean I/σ(I) obs: 3.5 / Rsym value: 0.259 / % possible all: 91.4 |
Reflection | *PLUS Num. measured all: 171366 / Rmerge(I) obs: 0.072 |
Reflection shell | *PLUS % possible obs: 91.4 % / Rmerge(I) obs: 0.259 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1MAL Resolution: 2.8→10 Å / Rfactor Rfree error: 0.0028 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED Cross valid method: THROUGHOUT EXCEPT FOR VERY LAST ROUND (REFINEMENT AGAINST ALL REFLECTIONS) σ(F): 0 Details: ALL SIDE CHAIN ATOMS WITHOUT DENSITY WERE ASSIGNED ZERO OCCUPANCY. B-VALUES OF WILSON PLOT WAS CALCULATED WITH THE CCP4 PROGRAM WILSON.
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Displacement parameters | Biso mean: 21.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati d res low obs: 3.56 Å / Luzzati sigma a obs: 0.38 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→10 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS / Rms dev Biso : 2 Å2 / Rms dev position: 0.2 Å / Weight Biso : 0.2 / Weight position: 100 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.8→2.9 Å / Rfactor Rfree error: 0.012 / Total num. of bins used: 10
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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