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- PDB-1mh6: Solution Structure of the Transposon Tn5-encoding Bleomycin-bindi... -

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Basic information

Entry
Database: PDB / ID: 1mh6
TitleSolution Structure of the Transposon Tn5-encoding Bleomycin-binding Protein, BLMT
ComponentsBLEOMYCIN RESISTANCE PROTEIN
KeywordsPROTEIN BINDING / Antibiotic resistance / Transposable element
Function / homology
Function and homology information


response to antibiotic
Similarity search - Function
Bleomycin resistance protein / 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1 / 2,3-Dihydroxybiphenyl 1,2-Dioxygenase; domain 1 / Glyoxalase/fosfomycin resistance/dioxygenase domain / Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily / Vicinal oxygen chelate (VOC) domain / Vicinal oxygen chelate (VOC) domain profile. / Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase / Roll / Alpha Beta
Similarity search - Domain/homology
Bleomycin resistance protein
Similarity search - Component
Biological speciesKlebsiella pneumoniae (bacteria)
MethodSOLUTION NMR
Model type detailsminimized average
AuthorsKumagai, T. / Ohtani, K. / Tsuboi, Y. / Koike, T. / Sugiyama, M.
CitationJournal: To be published
Title: Solution structure of the transposon Tn5-encoding bleomycin-binding protein complexed with an activated bleomycin analogue.
Authors: Kumagai, T. / Ohtani, K. / Tsuboi, Y. / Koike, T. / Sugiyama, M.
History
DepositionAug 19, 2002Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Feb 19, 2003Provider: repository / Type: Initial release
Revision 1.1Apr 28, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Data collection / Database references / Derived calculations
Category: database_2 / pdbx_nmr_software ...database_2 / pdbx_nmr_software / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name
Revision 1.4May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: BLEOMYCIN RESISTANCE PROTEIN
B: BLEOMYCIN RESISTANCE PROTEIN


Theoretical massNumber of molelcules
Total (without water)27,9072
Polymers27,9072
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
RepresentativeModel #1minimized average structure

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Components

#1: Protein BLEOMYCIN RESISTANCE PROTEIN / BLMT


Mass: 13953.740 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Klebsiella pneumoniae (bacteria) / Plasmid: pKKtrp / Production host: Escherichia coli (E. coli) / References: UniProt: P13081

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D NOESY
1212D TOCSY
131HMBC

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Sample preparation

Sample conditionsIonic strength: 0.20 / pH: 7.4 / Pressure: ambient / Temperature: 308 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometerType: JEOL ECP / Manufacturer: JEOL / Model: ECP / Field strength: 500 MHz

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Processing

NMR software
NameVersionClassification
Discover2000data analysis
Discover2000refinement
NMR representativeSelection criteria: minimized average structure
NMR ensembleConformers submitted total number: 1

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