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Open data
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Basic information
Entry | Database: PDB / ID: 1lbt | |||||||||
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Title | LIPASE (E.C.3.1.1.3) (TRIACYLGLYCEROL HYDROLASE) | |||||||||
![]() | LIPASE B | |||||||||
![]() | HYDROLASE (CARBOXYLIC ESTERASE) | |||||||||
Function / homology | ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Uppenberg, J. / Jones, T.A. | |||||||||
![]() | ![]() Title: Crystallographic and molecular-modeling studies of lipase B from Candida antarctica reveal a stereospecificity pocket for secondary alcohols. Authors: Uppenberg, J. / Ohrner, N. / Norin, M. / Hult, K. / Kleywegt, G.J. / Patkar, S. / Waagen, V. / Anthonsen, T. / Jones, T.A. #1: ![]() Title: Crystallization and Preliminary X-Ray Studies of Lipase B from Candida Antarctica Authors: Uppenberg, J. / Patkar, S. / Bergfors, T. / Jones, T.A. #2: ![]() Title: The Sequence, Crystal Structure Determination and Refinement of Two Crystal Forms of Lipase B from Candida Antarctica Authors: Uppenberg, J. / Hansen, M.T. / Patkar, S. / Jones, T.A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.2 KB | Display | ![]() |
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PDB format | ![]() | 95.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 70 / 2: CIS PROLINE - PRO 192 | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.0332, 0.0075, -0.9994), Vector ![]() Details | MTRIX THE TRANSFORMATIONS PRESENTED ON MTRIX RECORDS BELOW DESCRIBE NON-CRYSTALLOGRAPHIC RELATIONSHIPS AMONG THE VARIOUS DOMAINS IN THIS ENTRY. APPLYING THE APPROPRIATE MTRIX TRANSFORMATION TO THE RESIDUES LISTED FIRST WILL YIELD APPROXIMATE COORDINATES FOR THE RESIDUES LISTED SECOND. APPLIED TO TRANSFORMED TO MTRIX RESIDUES RESIDUES RMSD M1 A 1 .. A 317 ? 1 .. ? 317 | |
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Components
#1: Protein | Mass: 33040.238 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() #2: Polysaccharide | ![]() Source method: isolated from a genetically manipulated source #3: Chemical | #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.59 Å3/Da / Density % sol: 65.74 % | |||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | *PLUS Temperature: 20-25 ℃ / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 Å |
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Detector | Type: XUONG-HAMLIN MULTIWIRE MARK III / Detector: AREA DETECTOR / Date: Jun 18, 1990 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.5→50 Å / Num. obs: 26677 / % possible obs: 83 % / Redundancy: 1.7 % / Rmerge(I) obs: 0.041 |
Reflection | *PLUS % possible obs: 82 % / Observed criterion σ(I): 0 / Num. measured all: 45529 / Rmerge(I) obs: 0.041 |
Reflection shell | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 2.59 Å / % possible obs: 63 % / Rmerge(I) obs: 0.123 / Mean I/σ(I) obs: 6 |
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Processing
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Refinement | Resolution: 2.5→7.5 Å / σ(F): 2
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Displacement parameters | Biso mean: 19.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→7.5 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 2.59 Å |