+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1l3n | ||||||
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タイトル | The Solution Structure of Reduced Dimeric Copper Zinc SOD: the Structural Effects of Dimerization | ||||||
要素 | superoxide dismutase [Cu-Zn] | ||||||
キーワード | OXIDOREDUCTASE (酸化還元酵素) / reduced human copper/zinc superoxide dismutase / solution structure / homodimeric protein. | ||||||
機能・相同性 | 機能・相同性情報 action potential initiation / neurofilament cytoskeleton organization / positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / protein phosphatase 2B binding / regulation of organ growth / relaxation of vascular associated smooth muscle / response to superoxide / anterograde axonal transport / peripheral nervous system myelin maintenance / regulation of T cell differentiation in thymus ...action potential initiation / neurofilament cytoskeleton organization / positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / protein phosphatase 2B binding / regulation of organ growth / relaxation of vascular associated smooth muscle / response to superoxide / anterograde axonal transport / peripheral nervous system myelin maintenance / regulation of T cell differentiation in thymus / superoxide anion generation / retina homeostasis / negative regulation of cholesterol biosynthetic process / hydrogen peroxide biosynthetic process / auditory receptor cell stereocilium organization / retrograde axonal transport / regulation of protein kinase activity / myeloid cell homeostasis / muscle cell cellular homeostasis / regulation of GTPase activity / superoxide metabolic process / heart contraction / スーパーオキシドディスムターゼ / positive regulation of catalytic activity / Detoxification of Reactive Oxygen Species / transmission of nerve impulse / negative regulation of reproductive process / negative regulation of developmental process / superoxide dismutase activity / regulation of multicellular organism growth / response to axon injury / neuronal action potential / ectopic germ cell programmed cell death / positive regulation of phagocytosis / ovarian follicle development / axon cytoplasm / glutathione metabolic process / reactive oxygen species metabolic process / 着床 / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / dendrite cytoplasm / removal of superoxide radicals / : / positive regulation of superoxide anion generation / thymus development / regulation of mitochondrial membrane potential / locomotory behavior / positive regulation of cytokine production / determination of adult lifespan / placenta development / sensory perception of sound / response to hydrogen peroxide / ミトコンドリア / small GTPase binding / 血圧 / negative regulation of inflammatory response / ペルオキシソーム / Platelet degranulation / 遺伝子発現 / protein-folding chaperone binding / response to heat / cytoplasmic vesicle / 精子形成 / response to ethanol / intracellular iron ion homeostasis / negative regulation of neuron apoptotic process / positive regulation of MAPK cascade / ミトコンドリアマトリックス / response to xenobiotic stimulus / positive regulation of apoptotic process / copper ion binding / neuronal cell body / apoptotic process / protein-containing complex / ミトコンドリア / extracellular space / extracellular exosome / zinc ion binding / extracellular region / 核質 / identical protein binding / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing, restrained enegy minimization in vacuum | ||||||
データ登録者 | Banci, L. / Bertini, I. / Cramaro, F. / Del Conte, R. / Viezzoli, M.S. | ||||||
引用 | ジャーナル: Eur.J.Biochem. / 年: 2002 タイトル: The solution structure of reduced dimeric copper zinc superoxide dismutase. The structural effects of dimerization 著者: Banci, L. / Bertini, I. / Cramaro, F. / Del Conte, R. / Viezzoli, M.S. #1: ジャーナル: Biochemistry / 年: 2000 タイトル: Backbone dynamics of human Cu,Zn superoxide dismutase and of its monomeric F50E/G51E/E133Q mutant: the influence of dimerization on mobility and function 著者: Banci, L. / Bertini, I. / Cramaro, F. / Del Conte, R. / Rosato, A. / Viezzoli, M.S. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1l3n.cif.gz | 2.5 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1l3n.ent.gz | 2.2 MB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1l3n.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/l3/1l3n ftp://data.pdbj.org/pub/pdb/validation_reports/l3/1l3n | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 15779.430 Da / 分子数: 2 / 変異: C6A, C111S / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: SOD1 / プラスミド: pbr322 / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): TOPP1 参照: UniProt: P00441, スーパーオキシドディスムターゼ #2: 化合物 | #3: 化合物 | |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: Cu(I), Zn(II) SOD structure was determined using triple resonance three-and bi-dimensional and homonuclear bi-dimensional techniques. |
-試料調製
詳細 |
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試料状態 |
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-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M | ||||||||||||||||||||
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放射波長 | 相対比: 1 | ||||||||||||||||||||
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: simulated annealing, restrained enegy minimization in vacuum ソフトェア番号: 1 詳細: The structures were calculated using the constraints: 3566 NOE cross peaks were converted into distance constraints, 49 dihedral phi angles, 52 dihedral psi angles, 14 chi angles and 45 ...詳細: The structures were calculated using the constraints: 3566 NOE cross peaks were converted into distance constraints, 49 dihedral phi angles, 52 dihedral psi angles, 14 chi angles and 45 proton pairs stereospecifically assigned. The Cu(I) and Zn(II) metal ions were included in the calculation with special linkers. The disulfide bridge between Cys57 and Cys146 was introduced with upper and lower distance limits between the CB and SG atoms of the two side chains. | |||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structure with the lowest energy target function 計算したコンフォーマーの数: 1400 / 登録したコンフォーマーの数: 30 |