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Yorodumi- PDB-1kgd: Crystal Structure of the Guanylate Kinase-like Domain of Human CASK -
+Open data
-Basic information
Entry | Database: PDB / ID: 1kgd | ||||||
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Title | Crystal Structure of the Guanylate Kinase-like Domain of Human CASK | ||||||
Components | PERIPHERAL PLASMA MEMBRANE CASK | ||||||
Keywords | PROTEIN BINDING / MAGUK / CASK / GUANYLATE KINASE LIKE DOMAIN | ||||||
Function / homology | Function and homology information negative regulation of cellular response to growth factor stimulus / guanylate kinase activity / Dopamine Neurotransmitter Release Cycle / neurexin family protein binding / nuclear lamina / regulation of neurotransmitter secretion / negative regulation of wound healing / Assembly and cell surface presentation of NMDA receptors / calcium ion import / Neurexins and neuroligins ...negative regulation of cellular response to growth factor stimulus / guanylate kinase activity / Dopamine Neurotransmitter Release Cycle / neurexin family protein binding / nuclear lamina / regulation of neurotransmitter secretion / negative regulation of wound healing / Assembly and cell surface presentation of NMDA receptors / calcium ion import / Neurexins and neuroligins / Sensory processing of sound by outer hair cells of the cochlea / Sensory processing of sound by inner hair cells of the cochlea / Nephrin family interactions / ciliary membrane / regulation of synaptic vesicle exocytosis / Syndecan interactions / negative regulation of cell-matrix adhesion / positive regulation of calcium ion import / basement membrane / negative regulation of keratinocyte proliferation / establishment of localization in cell / Schaffer collateral - CA1 synapse / nuclear matrix / cell-cell junction / actin cytoskeleton / presynaptic membrane / basolateral plasma membrane / vesicle / calmodulin binding / non-specific serine/threonine protein kinase / cell adhesion / phosphorylation / signaling receptor binding / focal adhesion / protein serine kinase activity / protein serine/threonine kinase activity / nucleolus / positive regulation of transcription by RNA polymerase II / ATP binding / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.314 Å | ||||||
Authors | Li, Y. / Spangenberg, O. / Paarmann, I. / Konrad, M. / Lavie, A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: Structural basis for nucleotide-dependent regulation of membrane-associated guanylate kinase-like domains. Authors: Li, Y. / Spangenberg, O. / Paarmann, I. / Konrad, M. / Lavie, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1kgd.cif.gz | 92.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1kgd.ent.gz | 72.3 KB | Display | PDB format |
PDBx/mmJSON format | 1kgd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kg/1kgd ftp://data.pdbj.org/pub/pdb/validation_reports/kg/1kgd | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 20754.535 Da / Num. of mol.: 1 / Fragment: guanylate kinase like domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: O14936 | ||
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#2: Chemical | ChemComp-FMT / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 52.23 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: sodium formate, potassium chloride, magnesium chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-BM-D |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 22, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.31→50 Å / Num. obs: 48853 / % possible obs: 94.2 % / Redundancy: 6.7 % / Rsym value: 0.034 / Net I/σ(I): 20.9 |
Reflection shell | Resolution: 1.31→1.4 Å / Redundancy: 5.2 % / Mean I/σ(I) obs: 8.31 / Rsym value: 0.145 / % possible all: 81 |
Reflection | *PLUS Num. measured all: 327011 / Rmerge(I) obs: 0.034 |
Reflection shell | *PLUS % possible obs: 81 % / Rmerge(I) obs: 0.145 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.314→12 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.94 / SU B: 2.232 / SU ML: 0.04 / Isotropic thermal model: BABINET MODEL WITH MASK / Cross valid method: THROUGHOUT / ESU R: 0.07 / ESU R Free: 0.064 / Stereochemistry target values: Engh & Huber
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.768 Å2
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Refinement step | Cycle: LAST / Resolution: 1.314→12 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.314→1.348 Å / Total num. of bins used: 20
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Software | *PLUS Name: REFMAC / Version: 5 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS % reflection Rfree: 10.1 % / Rfactor obs: 0.186 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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