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Open data
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Basic information
Entry | Database: PDB / ID: 1k94 | ||||||
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Title | Crystal structure of des(1-52)grancalcin with bound calcium | ||||||
![]() | GRANCALCIN![]() | ||||||
![]() | METAL BINDING PROTEIN / penta-EF-hand protein / ![]() | ||||||
Function / homology | ![]() azurophil granule lumen / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jia, J. / Borregaard, N. / Lollike, K. / Cygler, M. | ||||||
![]() | ![]() Title: Structure of Ca(2+)-loaded human grancalcin. Authors: Jia, J. / Borregaard, N. / Lollike, K. / Cygler, M. #1: ![]() Title: Crystal structure of human grancalcin, a member of the Penta-EF-Hand Protein family Authors: Jia, J. / Han, Q. / Borregaard, N. / Lollike, K. / Cygler, M. #2: ![]() Title: Crystallizaiton and preliminary X-ray analysis of human grancalcin, a novel Ca2+-binding protein in leukocytes Authors: Han, Q. / Jia, J. / Li, Y. / Lollike, K. / Cygler, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83.7 KB | Display | ![]() |
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PDB format | ![]() | 61.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1k95C ![]() 1f4qS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 18771.225 Da / Num. of mol.: 2 / Fragment: residues 53-217 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.14 % |
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Crystal grow![]() | Temperature: 293 K / Method: evaporation / pH: 6 Details: PEG 8000, Calcium acetate, sodium cacodylate, pH 6.0, EVAPORATION at 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 12, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.7→50 Å / Num. all: 33645 / Num. obs: 30676 / % possible obs: 91.1 % / Redundancy: 4.7 % / Biso Wilson estimate: 16.2 Å2 / Rmerge(I) obs: 0.078 / Rsym value: 0.064 / Net I/σ(I): 11.1 |
Reflection shell | Resolution: 1.7→1.76 Å / Rmerge(I) obs: 0.332 / Rsym value: 0.331 / % possible all: 82.4 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1F4Q Resolution: 1.7→50 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.7→50 Å
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Refine LS restraints |
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