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- PDB-1k1a: Crystal structure of the ankyrin repeat domain of Bcl-3: a unique... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1k1a | ||||||
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Title | Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family | ||||||
![]() | B-cell lymphoma 3-encoded protein | ||||||
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Function / homology | ![]() Bcl3-Bcl10 complex / follicular dendritic cell differentiation / Bcl3/NF-kappaB2 complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Michel, F. / Soler-Lopez, M. / Petosa, C. / Cramer, P. / Siebenlist, U. / Mueller, C.W. | ||||||
![]() | ![]() Title: Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family. Authors: Michel, F. / Soler-Lopez, M. / Petosa, C. / Cramer, P. / Siebenlist, U. / Muller, C.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 59.7 KB | Display | ![]() |
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PDB format | ![]() | 43.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1k1bC ![]() 1iknS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25829.451 Da / Num. of mol.: 1 / Fragment: ankyrin repeat domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.12 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG 6000, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 5.8 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 28, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→40 Å / Num. all: 17021 / Num. obs: 17021 / % possible obs: 89.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.7 % / Rsym value: 0.072 / Net I/σ(I): 10.5 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 1.73 % / Mean I/σ(I) obs: 1.65 / Num. unique all: 1479 / Rsym value: 0.697 / % possible all: 77.8 |
Reflection | *PLUS Lowest resolution: 40 Å / Redundancy: 2.71 % / Num. measured all: 46088 / Rmerge(I) obs: 0.072 |
Reflection shell | *PLUS % possible obs: 77.8 % / Rmerge(I) obs: 0.697 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1ikn Resolution: 1.86→20 Å / SU B: 3.68115 / SU ML: 0.11055 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.18488 / ESU R Free: 0.15154 / Stereochemistry target values: Engh & Huber Details: RESIDUES SER178,VAL179,LEU182,GLN251,ARG269,SER307 PRESENT DOUBLE CONFORMATIONS.
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Displacement parameters | Biso mean: 21.105 Å2
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Refinement step | Cycle: LAST / Resolution: 1.86→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor obs: 0.199 | ||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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