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Yorodumi- PDB-1k0s: Solution structure of the chemotaxis protein CheW from the thermo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1k0s | ||||||
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Title | Solution structure of the chemotaxis protein CheW from the thermophilic organism Thermotoga maritima | ||||||
Components | CHEMOTAXIS PROTEIN CHEW | ||||||
Keywords | SIGNALING PROTEIN / CheW | ||||||
Function / homology | Function and homology information chemotaxis / protein domain specific binding / signal transduction / cytosol Similarity search - Function | ||||||
Biological species | Thermotoga maritima (bacteria) | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing | ||||||
Authors | Griswold, I.J. / Zhou, H. / Swanson, R.V. / Simon, M.I. / Dahlquist, F.W. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2002 Title: The solution structure and interactions of CheW from Thermotoga maritima. Authors: Griswold, I.J. / Zhou, H. / Matison, M. / Swanson, R.V. / McIntosh, L.P. / Simon, M.I. / Dahlquist, F.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1k0s.cif.gz | 949.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1k0s.ent.gz | 820.6 KB | Display | PDB format |
PDBx/mmJSON format | 1k0s.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k0/1k0s ftp://data.pdbj.org/pub/pdb/validation_reports/k0/1k0s | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16977.836 Da / Num. of mol.: 1 / Fragment: CheW Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermotoga maritima (bacteria) / Gene: CheW / Plasmid: pCW / Production host: Escherichia coli (E. coli) / Strain (production host): 594 / References: UniProt: Q56311 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details | Contents: 1mM TMW U-15N,13C; 50mM Acetate buffer / Solvent system: 93% H2O/7% D2O |
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Sample conditions | Ionic strength: 50mM KCl / pH: 4.5 / Pressure: 1 atm / Temperature: 308 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |