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- PDB-1jsv: The structure of cyclin-dependent kinase 2 (CDK2) in complex with... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jsv | ||||||
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Title | The structure of cyclin-dependent kinase 2 (CDK2) in complex with 4-[(6-amino-4-pyrimidinyl)amino]benzenesulfonamide | ||||||
![]() | CELL DIVISION PROTEIN KINASE 2![]() | ||||||
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Function / homology | ![]() cyclin A1-CDK2 complex / cyclin E2-CDK2 complex / cyclin E1-CDK2 complex / cyclin A2-CDK2 complex / positive regulation of DNA-templated DNA replication initiation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Watenpaugh, K.D. / Kelley, L.C. | ||||||
![]() | ![]() Title: The cyclin-dependent kinases cdk2 and cdk5 act by a random, anticooperative kinetic mechanism Authors: Clare, P.M. / Poorman, R.A. / Kelley, L.C. / Watenpaugh, K.D. / Bannow, C.A. / Leach, K.L. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.2 KB | Display | ![]() |
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PDB format | ![]() | 58.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 33976.488 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P24941, ![]() |
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#2: Chemical | ChemComp-U55 / |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.53 % | ||||||||||||||||||||
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.3 Details: 200mM HEPES, beta-mercaptoethanal, pH 7.30, VAPOR DIFFUSION, SITTING DROP, temperature 277K | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 5 ℃ / pH: 7.4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() | |||||||||
Detector | Type: SIEMENS HI-STAR / Detector: AREA DETECTOR / Details: MONOCHROMATOR | |||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.96→10 Å / Num. obs: 18495 / % possible obs: 91.4 % / Observed criterion σ(I): 2 / Redundancy: 3.88 % / Rmerge(I) obs: 0.049 | |||||||||
Reflection shell | Resolution: 1.96→2.03 Å / Rmerge(I) obs: 0.18 / Mean I/σ(I) obs: 4.4 / % possible all: 34 |
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Processing
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Refinement | Method to determine structure![]() | ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.96→10 Å
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 10 Å / σ(F): 2 / Rfactor all![]() | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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