- PDB-1jnj: NMR solution structure of the human beta2-microglobulin -
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IDまたはキーワード:
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基本情報
登録情報
データベース: PDB / ID: 1jnj
タイトル
NMR solution structure of the human beta2-microglobulin
要素
beta2-microglobulin
キーワード
IMMUNE SYSTEM (免疫系) / immunoglobulin constant domain
機能・相同性
機能・相同性情報
positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / cellular response to iron(III) ion ...positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / ER to Golgi transport vesicle membrane / regulation of erythrocyte differentiation / peptide antigen assembly with MHC class I protein complex / response to molecule of bacterial origin / regulation of iron ion transport / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / cellular response to iron ion / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / negative regulation of neurogenesis / peptide antigen assembly with MHC class II protein complex / positive regulation of receptor-mediated endocytosis / positive regulation of T cell mediated cytotoxicity / MHC class II protein complex / cellular response to nicotine / recycling endosome membrane / specific granule lumen / phagocytic vesicle membrane / peptide antigen binding / positive regulation of cellular senescence / antigen processing and presentation of exogenous peptide antigen via MHC class II / negative regulation of epithelial cell proliferation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / Modulation by Mtb of host immune system / sensory perception of smell / positive regulation of T cell activation / positive regulation of protein binding / tertiary granule lumen / DAP12 signaling / negative regulation of neuron projection development / MHC class II protein complex binding / late endosome membrane / T cell differentiation in thymus / ER-Phagosome pathway / iron ion transport / early endosome membrane / protein refolding / protein homotetramerization / intracellular iron ion homeostasis / amyloid fibril formation / learning or memory / Amyloid fiber formation / lysosomal membrane / external side of plasma membrane / 小胞体 / ゴルジ体 / focal adhesion / Neutrophil degranulation / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / ゴルジ体 / 小胞体 / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / 生体膜 / identical protein binding / 細胞膜 / 細胞質基質 類似検索 - 分子機能
Text: This structure was determined using standard 2D homonuclear techniques
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試料調製
詳細
Solution-ID
内容
溶媒系
1
0.68 mM beta2-m; 70 mM phosphate buffer NA, 100 mM NaCl.
90% H2O/10% D2O
2
0.37 mM beta2-m; 70 mM phosphate buffer NA, 100 mM NaCl.
100% D2O
試料状態
Conditions-ID
イオン強度
pH
圧 (kPa)
温度 (K)
1
100mMNaCl, 70mMphosphatebuffer
6.4
ambient
310K
2
100mMNaCl, 70mMphosphatebuffer
6.4
ambient
310K
結晶化
*PLUS
手法: その他 / 詳細: NMR
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 500 MHz
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解析
NMR software
名称
バージョン
開発者
分類
XwinNMR
2.6
Bruker
collection
Felix
2000
MSI
解析
Felix
2000
MSI
データ解析
DIANA
1.5
Guentert
構造決定
Discover
2000
MSI
精密化
精密化
手法: simulated annealing, restrained molecular dynamics, restrained molecular mechanics ソフトェア番号: 1 詳細: the structures are based on a total of 1608 restraints, 1541 are NOE-derived distance contraints, 67 dihedral angle restraints
NMRアンサンブル
コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 380 / 登録したコンフォーマーの数: 20