[English] 日本語
Yorodumi- PDB-1jja: CRYSTAL STRUCTURE OF ORTHORHOMBIC FORM OF D90E MUTANT OF ESCHERIC... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1jja | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF ORTHORHOMBIC FORM OF D90E MUTANT OF ESCHERICHIA COLI L-ASPARAGINASE II | ||||||
Components | L-ASPARAGINASE IIAsparaginase | ||||||
Keywords | HYDROLASE / L-ASPARAGINASE / LEUKEMIA | ||||||
Function / homology | Function and homology information asparagine catabolic process / asparaginase / asparaginase activity / outer membrane-bounded periplasmic space / protein homotetramerization / periplasmic space / protein-containing complex / identical protein binding Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Borek, D. / Kozak, M. / Jaskolski, M. | ||||||
Citation | Journal: Febs J. / Year: 2014 Title: Crystal structure of active site mutant of antileukemic L-asparaginase reveals conserved zinc-binding site. Authors: Borek, D. / Kozak, M. / Pei, J. / Jaskolski, M. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1993 Title: Crystal Structure of Escherichia coli L-asparaginase, An Enzyme Used in Cancer Therapy Authors: Swain, A.L. / Jaskolski, M. / Housset, D. / Rao, J.K. / Wlodawer, A. #2: Journal: FEBS Lett. / Year: 1996 Title: A Covalently Bound Catalytic Intermediate in Escherichia coli Asparaginase: Crystal Structure of a Thr-89-Val Mutant Authors: Palm, G.J. / Lubkowski, J. / Derst, C. / Schleper, S. / Rohm, K.H. / Wlodawer, A. #3: Journal: Acta Crystallogr.,Sect.D / Year: 2001 Title: Structures of Two Highly Homologous Bacterial L-Asparaginases: A Case of Enantiomorphic Space Groups Authors: Jaskolski, M. / Kozak, M. / Lubkowski, J. / Palm, G. / Wlodawer, A. #4: Journal: ACTA BIOCHIM.POL. / Year: 1997 Title: Why a "benign" Mutation Kills Enzyme Activity. Structure-based Analysis of the A176V Mutant of Saccharomyces cerevisiae L-asparaginase I Authors: Bonthron, D.T. / Jaskolski, M. #5: Journal: BIOCHIM.BIOPHYS.ACTA / Year: 2000 Title: Dynamics of a Mobile Loop at the Active Site of Escherichia coli Asparaginase Authors: Aung, H.P. / Bocola, M. / Schleper, S. / Rohm, K.H. | ||||||
History |
| ||||||
Remark 300 | BIOMOLECULE: 1, 2 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 6 ...BIOMOLECULE: 1, 2 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 6 CHAINS, A, B, C, D, E, F. THE BIOLOGICAL ASSEMBLY IS A HOMOTETRAMER. CHAINS A, B, C, D FORM ONE INDEPENDENT TETRAMER WITH NON-CRYSTALLOGRAPHIC 222 SYMMETRY. CHAINS E AND F FORM AN ACTIVE-SITE-COMPETENT DIMER (CORRESPONDING TO DIMER AC IN THE ABCD TETRAMER). THE COMPLETE EFE'F' TETRAMER IS GENERATED THROUGH THE CRYSTALLOGRAPHIC TWO-FOLD ROTATION. SEE REMARK 350 FOR INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S). |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1jja.cif.gz | 355.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1jja.ent.gz | 291.9 KB | Display | PDB format |
PDBx/mmJSON format | 1jja.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jj/1jja ftp://data.pdbj.org/pub/pdb/validation_reports/jj/1jja | HTTPS FTP |
---|
-Related structure data
Related structure data | 1ihdC 1jazC 3ecaS C: citing same article (ref.) S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
| ||||||||
Details | The biological assembly is a homotetramer. The asymmetric unit contains six protein chains, A, B, C, D, E, F. Chains A, B, C, D form one independent tetramer with non-crystallographic 222 symmetry. Chains E and F form an active-site-competent dimer (corresponding to dimer AC in the ABCD tetramer). The complete EFE'F' tetramer is generated through the crystallographic two-fold rotation. |
-Components
#1: Protein | Mass: 34640.836 Da / Num. of mol.: 6 / Mutation: D90E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: UniProt: P00805, asparaginase #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43 % |
---|---|
Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 9 Details: PEG MME550, bicine, NaCl , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 290 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: SIEMENS / Wavelength: 1.54178 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 10, 1998 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→25 Å / Num. all: 75961 / Num. obs: 75961 / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.14 % / Rmerge(I) obs: 0.123 / Net I/σ(I): 11.2 |
Reflection shell | Resolution: 2.3→2.41 Å / Redundancy: 3.07 % / Rmerge(I) obs: 0.813 / Mean I/σ(I) obs: 1.8 / % possible all: 93.9 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Active dimer of native E.coli asparaginase II (PDB code: 3ECA) Resolution: 2.3→10 Å / SU B: 5.781 / SU ML: 0.136 / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.40726 / ESU R Free: 0 Details: MAXIMUM LIKELIHOOD METHOD. RESIDUES 17-33 IN MONOMER A, 16-37 IN MONOMER B, 16-34 IN MONOMER D, 16-34 IN MONOMER E, 19-20 AND 30-33 IN MONOMER F ARE NOT PRESENT IN THE MODEL. IN MONOMER C, ...Details: MAXIMUM LIKELIHOOD METHOD. RESIDUES 17-33 IN MONOMER A, 16-37 IN MONOMER B, 16-34 IN MONOMER D, 16-34 IN MONOMER E, 19-20 AND 30-33 IN MONOMER F ARE NOT PRESENT IN THE MODEL. IN MONOMER C, THE COMPLETE FLEXIBLE LOOP BETWEEN RESIDUES 10 AND 40 HAS BEEN MODELED IN VERY GOOD ELECTRON DENSITY IN AN OPEN CONFORMATION.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.399 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|