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Open data
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Basic information
Entry | Database: PDB / ID: 1jhg | ||||||
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Title | TRP REPRESSOR MUTANT V58I | ||||||
![]() | TRP OPERON REPRESSOR | ||||||
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Function / homology | ![]() sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lawson, C.L. | ||||||
![]() | ![]() Title: An atomic view of the L-tryptophan binding site of trp repressor. Authors: Lawson, C.L. #1: Journal: Biological Structure and Dynamics: Proceedings of the Ninth Conversation in the Discipline Biomolecular Stereodynamics, Held at the State University of New York at Albany, June 20-24, 1995 Year: 1996 Title: Structural Consequences of Twp Methyl Additions in the Escherichia Coli Trp Repressor L-Tryptophan Binding Pocket Authors: Lawson, C.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58.1 KB | Display | ![]() |
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PDB format | ![]() | 43.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 11578.260 Da / Num. of mol.: 1 / Mutation: CHAIN A, V58I Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Description: I58 TRP REPRESSOR WAS GROWN IN ESCHERICHIA COLI STRAIN BL21 (DE3) USING T7 EXPRESSION SYSTEM VECTORS; Cell line: BL21 / Plasmid: PET13A / Species (production host): Escherichia coli / Cell (production host): CYTOPLASM / Gene (production host): TRPRI58 / Production host: ![]() ![]() ![]() |
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#2: Chemical | ChemComp-PO4 / ![]() |
#3: Chemical | ChemComp-TRP / ![]() |
#4: Water | ChemComp-HOH / ![]() |
Compound details | TWO TORSIONAL ANGLES IN THE L-TRYPTOPHAN BINDING POCKET HAVE DISTINCTLY NON-IDEAL GEOMETRY: CHI1 OF ...TWO TORSIONAL ANGLES IN THE L-TRYPTOPHAN |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 37 % | ||||||||||||||||||||
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Crystal grow![]() | Method: vapor diffusion, hanging drop / pH: 5 Details: HANGING DROP VAPOR DIFFUSION 2.0 M SODIUM PHOSPHATE, PH 5.0 600 MM AMMONIUM CHLORIDE 5 MM L-TRYPTOPHAN, vapor diffusion - hanging drop | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE AREA DETECTOR / Date: Aug 1, 1994 |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.3→30 Å / Num. obs: 86193 / % possible obs: 97.4 % / Observed criterion σ(I): 0.25 / Redundancy: 3.6 % / Rmerge(I) obs: 0.032 / Net I/σ(I): 28.4 |
Reflection shell | Resolution: 1.3→1.35 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.167 / Mean I/σ(I) obs: 20 / % possible all: 94 |
Reflection shell | *PLUS % possible obs: 94 % |
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Processing
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Refinement | Method to determine structure![]() Resolution: 1.3→30 Å / Num. parameters: 8337 / Num. restraintsaints: 2568 / Cross valid method: FREE R / Stereochemistry target values: ENGH AND HUBER Details: RIDING HYDROGEN ATOM OPTION OF SHELXL USED, ANISOTROPIC TEMPERATURE FACTORS REFINED BUT NOT INCLUDED IN THE DEPOSITION. SOME SIDE-CHAIN, SOLVENT, AND ALL PHOSPHATE ATOMS HAVE PARTIAL ...Details: RIDING HYDROGEN ATOM OPTION OF SHELXL USED, ANISOTROPIC TEMPERATURE FACTORS REFINED BUT NOT INCLUDED IN THE DEPOSITION. SOME SIDE-CHAIN, SOLVENT, AND ALL PHOSPHATE ATOMS HAVE PARTIAL OCCUPANCIES. APPARENT "SHORT" INTERATOMIC DISTANCES INDICATE POSITIONS IN THE CRYSTAL WHERE THERE ARE AT LEAST TWO ALTERNATE MODELS.
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Solvent computation | Solvent model: SWAT 7.731 2.0 (BABINET'S PRINCIPLE) | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 15 / Occupancy sum hydrogen: 4248 / Occupancy sum non hydrogen: 3504 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.3→30 Å
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Refine LS restraints |
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