[English] 日本語
Yorodumi- PDB-1jc6: SOLUTION STRUCTURE OF BUNGARUS FACIATUS IX, A KUNITZ-TYPE CHYMOTR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1jc6 | ||||||
---|---|---|---|---|---|---|---|
Title | SOLUTION STRUCTURE OF BUNGARUS FACIATUS IX, A KUNITZ-TYPE CHYMOTRYPSIN INHIBITOR | ||||||
Components | VENOM BASIC PROTEASE INHIBITORS IX AND VIIIB | ||||||
Keywords | TOXIN / snake venom / Kunitz inhibitor / protease inhibitor / neurotoxin / solution structure / BF IX / Chymotrypsin Inhibitor | ||||||
Function / homology | Function and homology information serine-type endopeptidase inhibitor activity / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | Bungarus fasciatus (banded krait) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Chen, C. / Hsu, C.H. / Su, N.Y. / Chiou, S.H. / Wu, S.H. | ||||||
Citation | Journal: J.BIOL.CHEM. / Year: 2001 Title: Solution structure of a Kunitz-type chymotrypsin inhibitor isolated from the elapid snake Bungarus fasciatus Authors: Chen, C. / Hsu, C.H. / Su, N.Y. / Lin, Y.C. / Chiou, S.H. / Wu, S.H. #1: Journal: INT.J.PEPT.PROTEIN RES. / Year: 1983 Title: Complete amino acid sequences of two protease inhibitors in the venom of Bungarus fasciatus Authors: Liu, C.S. / Wu, T.C. / Lo, T.B. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1jc6.cif.gz | 197 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1jc6.ent.gz | 169.5 KB | Display | PDB format |
PDBx/mmJSON format | 1jc6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1jc6_validation.pdf.gz | 343.4 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1jc6_full_validation.pdf.gz | 408 KB | Display | |
Data in XML | 1jc6_validation.xml.gz | 12.8 KB | Display | |
Data in CIF | 1jc6_validation.cif.gz | 20.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jc/1jc6 ftp://data.pdbj.org/pub/pdb/validation_reports/jc/1jc6 | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 7305.265 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bungarus fasciatus (banded krait) / Tissue: VENOM / References: UniProt: P25660 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||
NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 2mM BF IX; 50mM phosphate buffer NA; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample conditions |
| ||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
|
-Processing
NMR software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 599 restraints, 536 are NOE-derived distance constraints, 33 dihedral angle restraints, 30 distance restraints from hydrogen bonds. | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are the 10 structures with the lowest Conformers calculated total number: 90 / Conformers submitted total number: 10 |