+Open data
-Basic information
Entry | Database: PDB / ID: 1j4n | ||||||
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Title | Crystal Structure of the AQP1 water channel | ||||||
Components | AQUAPORIN 1Aquaporin-1 | ||||||
Keywords | MEMBRANE PROTEIN / CHANNEL PROTEIN / TRANSMEMBRANE HELICES | ||||||
Function / homology | Function and homology information : / dense core granule membrane / Passive transport by Aquaporins / ammonium transmembrane transport => GO:0072488 / nitric oxide transmembrane transporter activity / metanephric descending thin limb development / metanephric proximal straight tubule development / metanephric proximal convoluted tubule segment 2 development / metanephric glomerulus vasculature development / Erythrocytes take up carbon dioxide and release oxygen ...: / dense core granule membrane / Passive transport by Aquaporins / ammonium transmembrane transport => GO:0072488 / nitric oxide transmembrane transporter activity / metanephric descending thin limb development / metanephric proximal straight tubule development / metanephric proximal convoluted tubule segment 2 development / metanephric glomerulus vasculature development / Erythrocytes take up carbon dioxide and release oxygen / Erythrocytes take up oxygen and release carbon dioxide / lipid digestion / cellular response to salt stress / renal water transport / glycerol transmembrane transporter activity / corticotropin secretion / secretory granule organization / carbon dioxide transmembrane transport / carbon dioxide transmembrane transporter activity / symbiont-containing vacuole / renal water absorption / positive regulation of saliva secretion / glycerol transmembrane transport / water transmembrane transporter activity / establishment or maintenance of actin cytoskeleton polarity / lateral ventricle development / cellular response to mercury ion / water channel activity / intracellular water homeostasis / cellular response to inorganic substance / intracellularly cGMP-activated cation channel activity / water transport / transepithelial water transport / glomerular filtration / response to xenobiotic stimulus => GO:0009410 / ammonium transmembrane transporter activity / camera-type eye morphogenesis / cellular homeostasis / cellular hyperosmotic response / hyperosmotic response / cell volume homeostasis / nitric oxide transport / Vasopressin regulates renal water homeostasis via Aquaporins / cGMP-mediated signaling / brush border / potassium channel activity / transmembrane transporter activity / cellular response to retinoic acid / cellular response to cAMP / sensory perception of pain / cellular response to copper ion / ephrin receptor binding / cellular response to dexamethasone stimulus / basal plasma membrane / caveola / brush border membrane / sarcolemma / carbon dioxide transport / wound healing / cellular response to hydrogen peroxide / cellular response to mechanical stimulus / positive regulation of angiogenesis / cellular response to UV / positive regulation of fibroblast proliferation / apical part of cell / cellular response to hypoxia / basolateral plasma membrane / nuclear membrane / membrane => GO:0016020 / positive regulation of cell migration / apical plasma membrane / axon / negative regulation of apoptotic process / extracellular exosome / nucleus / plasma membrane Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.2 Å | ||||||
Authors | Sui, H. / Han, B.-G. / Lee, J.K. / Walian, P. / Jap, B.K. | ||||||
Citation | Journal: Nature / Year: 2001 Title: Structural basis of water-specific transport through the AQP1 water channel. Authors: Sui, H. / Han, B.G. / Lee, J.K. / Walian, P. / Jap, B.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1j4n.cif.gz | 62.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1j4n.ent.gz | 46.3 KB | Display | PDB format |
PDBx/mmJSON format | 1j4n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j4/1j4n ftp://data.pdbj.org/pub/pdb/validation_reports/j4/1j4n | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 28824.377 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Tissue: blood / References: UniProt: P47865 | ||
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#2: Sugar | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 3.41 Å3/Da / Density % sol: 63.91 % Description: Data collected at ALS was used for final data processing. Data collected at NSLS was used for initial data processing. | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: evaporation / pH: 7.5 Details: PEG, monomethyl ether 550, Tris-HCl, NG, pH 7.5, EVAPORATION, temperature 277K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop / Details: Sui, H., (2000) Acta Crystallogr., D56, 1198. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Diffraction source |
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Detector | Type: ADSC / Detector: CCD | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 1.0367 Å / Relative weight: 1 | |||||||||||||||
Reflection | Resolution: 2.2→15 Å / Num. all: 20605 / Num. obs: 19626 / % possible obs: 95.2 % / Observed criterion σ(I): 0 / Redundancy: 15.8 % / Biso Wilson estimate: 42.7 Å2 / Rmerge(I) obs: 0.062 / Net I/σ(I): 41.8 | |||||||||||||||
Reflection shell | Resolution: 2.2→2.34 Å / Rmerge(I) obs: 0.58 / Num. unique all: 2450 / % possible all: 94.6 | |||||||||||||||
Reflection | *PLUS % possible obs: 99.7 % / Rmerge(I) obs: 0.062 | |||||||||||||||
Reflection shell | *PLUS Lowest resolution: 2.3 Å / % possible obs: 100 % / Rmerge(I) obs: 0.582 / Mean I/σ(I) obs: 5.3 |
-Processing
Software |
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Refinement | Resolution: 2.2→15 Å / Rfactor Rfree error: 0.009 / Cross valid method: THROUGHOUT / σ(F): 2
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 95.768 Å2 / ksol: 0.32175 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 53.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.031 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 15 Å / Rfactor obs: 0.266 / Rfactor Rfree: 0.308 / Rfactor Rwork: 0.266 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.393 / Rfactor Rwork: 0.36 |