[English] 日本語
Yorodumi- PDB-1ijj: THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ijj | ||||||
---|---|---|---|---|---|---|---|
Title | THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION | ||||||
Components | ACTIN, ALPHA SKELETAL MUSCLE | ||||||
Keywords | CONTRACTILE PROTEIN / actin / latrunculin / cytoskeleton | ||||||
Function / homology | Function and homology information cytoskeletal motor activator activity / tropomyosin binding / myosin heavy chain binding / mesenchyme migration / troponin I binding / actin filament bundle / filamentous actin / actin filament bundle assembly / skeletal muscle thin filament assembly / striated muscle thin filament ...cytoskeletal motor activator activity / tropomyosin binding / myosin heavy chain binding / mesenchyme migration / troponin I binding / actin filament bundle / filamentous actin / actin filament bundle assembly / skeletal muscle thin filament assembly / striated muscle thin filament / skeletal muscle myofibril / actin monomer binding / skeletal muscle fiber development / stress fiber / titin binding / actin filament polymerization / filopodium / actin filament / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / calcium-dependent protein binding / lamellipodium / cell body / hydrolase activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / magnesium ion binding / ATP binding / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Oryctolagus cuniculus (rabbit) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.85 Å | ||||||
Authors | Vorobiev, S.M. / Bubb, M.R. / Almo, S.C. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution Authors: Bubb, M.R. / Govindasamy, L. / Yarmola, E.G. / Vorobiev, S.M. / Almo, S.C. / Somasundaram, T. / Chapman, M.S. / Agbandje-McKenna, M. / McKenna, R. #1: Journal: J.Biol.Chem. / Year: 2000 Title: Actin-latrunculin A structure and function. Differential modulation of actin-binding protein function by latrunculin A Authors: Yarmola, E.G. / Somasundaram, T. / Boring, T.A. / Spector, I. / Bubb, M.R. #2: Journal: Nat.Cell Biol. / Year: 2000 Title: Latrunculin alters the actin-monomer subunit interface to prevent polymerization Authors: Morton, W.M. / Ayscough, K.R. / McLaughlin, P.J. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1ijj.cif.gz | 158.8 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1ijj.ent.gz | 121 KB | Display | PDB format |
PDBx/mmJSON format | 1ijj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ij/1ijj ftp://data.pdbj.org/pub/pdb/validation_reports/ij/1ijj | HTTPS FTP |
---|
-Related structure data
Related structure data | 1lcuC 1yagS S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||
2 |
| ||||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 42096.953 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Oryctolagus cuniculus (rabbit) / Tissue: MUSCLESkeletal muscle / References: UniProt: P68135 #2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.78 Å3/Da / Density % sol: 67.46 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.8 Details: ammonium sulfate, magnesium chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP at 298 K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X9B / Wavelength: 0.984 Å |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.984 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→30 Å / Num. obs: 29997 / % possible obs: 98.7 % / Redundancy: 4 % / Rmerge(I) obs: 0.049 |
Reflection shell | Resolution: 2.85→2.95 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.527 / Num. unique all: 2969 / % possible all: 99.3 |
-Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1YAG Resolution: 2.85→15 Å / Isotropic thermal model: RESTRAINED / σ(F): 2
| |||||||||||||||||||||||||
Displacement parameters |
| |||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.48 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.85→15 Å
| |||||||||||||||||||||||||
Refine LS restraints |
|