+Open data
-Basic information
Entry | Database: PDB / ID: 1iai | ||||||
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Title | IDIOTYPE-ANTI-IDIOTYPE FAB COMPLEX | ||||||
Components |
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Keywords | COMPLEX (IMMUNOGLOBULIN IGG1/IGG2A) / COMPLEX (IMMUNOGLOBULIN IGG1-IGG2A) / COMPLEX (IMMUNOGLOBULIN IGG1-IGG2A) complex | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.9 Å | ||||||
Authors | Ban, N. / Escobar, C. / Garcia, R. / Hasel, K. / Day, J. / Greenwood, A. / McPherson, A. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 Title: Crystal structure of an idiotype-anti-idiotype Fab complex. Authors: Ban, N. / Escobar, C. / Garcia, R. / Hasel, K. / Day, J. / Greenwood, A. / McPherson, A. #1: Journal: J.Mol.Biol. / Year: 1993 Title: Preliminary Crystallographic Study of a Complex between an Fab of a Monoclonal Feline Peritonitis Virus Neutralizing Antibody and its Anti-Idiotype Fab Authors: Ban, N. / Escobar, C. / Day, J. / Greenwood, A. / McPherson, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1iai.cif.gz | 169.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1iai.ent.gz | 133.5 KB | Display | PDB format |
PDBx/mmJSON format | 1iai.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ia/1iai ftp://data.pdbj.org/pub/pdb/validation_reports/ia/1iai | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO L 141 / 2: CIS PROLINE - PRO H 155 / 3: CIS PROLINE - PRO M 8 / 4: CIS PROLINE - PRO M 96 / 5: CIS PROLINE - PRO M 142 |
-Components
#1: Antibody | Mass: 23834.291 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: GenBank: 10121892 |
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#2: Antibody | Mass: 23679.373 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) |
#3: Antibody | Mass: 23560.805 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: GenBank: 1042224, UniProt: P01837*PLUS |
#4: Antibody | Mass: 23812.816 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: GenBank: 1042226 |
Sequence details | FAB 730.1.4: LIGHT CHAIN VARIABLE REGION. SEQUENTIAL RESIDUE NUMBERS KABAT RESIDUE NUMBERS 1 - 107 ...FAB 730.1.4: LIGHT CHAIN VARIABLE REGION. SEQUENTIAL |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.92 % | |||||||||||||||
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Crystal | *PLUS Density % sol: 57 % | |||||||||||||||
Crystal grow | *PLUS Temperature: 23 ℃ / pH: 7 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.9→40 Å / Num. obs: 23640 / % possible obs: 92 % / Observed criterion σ(I): 0 |
Reflection | *PLUS Num. measured all: 89898 / Rmerge(I) obs: 0.112 / Biso Wilson estimate: 13 Å2 |
-Processing
Software |
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Refinement | Resolution: 2.9→40 Å / σ(I): 3
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Refinement step | Cycle: LAST / Resolution: 2.9→40 Å
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Refine LS restraints |
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Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 15 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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