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- PDB-1ho7: NMR STRUCTURE OF THE POTASSIUM CHANNEL FRAGMENT L45 IN TFE -

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Basic information

Entry
Database: PDB / ID: 1ho7
TitleNMR STRUCTURE OF THE POTASSIUM CHANNEL FRAGMENT L45 IN TFE
ComponentsVOLTAGE-GATED POTASSIUM CHANNEL PROTEIN
KeywordsMEMBRANE PROTEIN / helix
Function / homology
Function and homology information


mating behavior, sex discrimination / Phase 3 - rapid repolarisation / behavioral response to ether / Voltage gated Potassium channels / proboscis extension reflex / larval locomotory behavior / regulation of synaptic activity / courtship behavior / positive regulation of membrane potential / positive regulation of circadian sleep/wake cycle, sleep ...mating behavior, sex discrimination / Phase 3 - rapid repolarisation / behavioral response to ether / Voltage gated Potassium channels / proboscis extension reflex / larval locomotory behavior / regulation of synaptic activity / courtship behavior / positive regulation of membrane potential / positive regulation of circadian sleep/wake cycle, sleep / regulation of circadian sleep/wake cycle, sleep / detection of visible light / delayed rectifier potassium channel activity / sleep / cellular response to dopamine / voltage-gated monoatomic cation channel activity / axon extension / action potential / voltage-gated potassium channel activity / voltage-gated potassium channel complex / potassium ion transmembrane transport / protein homooligomerization / potassium ion transport / sensory perception of taste / learning or memory / membrane
Similarity search - Function
Potassium channel, voltage dependent, Kv1 / Potassium channel, voltage dependent, Kv / Potassium channel tetramerisation-type BTB domain / BTB/POZ domain / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / Voltage-dependent channel domain superfamily / SKP1/BTB/POZ domain superfamily / Ion transport domain / Ion transport protein
Similarity search - Domain/homology
Potassium voltage-gated channel protein Shaker
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
MethodSOLUTION NMR / Hybrid distance geometry, simulated annealing
AuthorsOhlenschlager, O. / Hojo, H. / Ramachandran, R. / Gorlach, M. / Haris, P.I.
CitationJournal: Biophys.J. / Year: 2002
Title: Three-dimensional structure of the S4-S5 segment of the Shaker potassium channel.
Authors: Ohlenschlager, O. / Hojo, H. / Ramachandran, R. / Gorlach, M. / Haris, P.I.
History
DepositionDec 10, 2000Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 5, 2002Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN


Theoretical massNumber of molelcules
Total (without water)2,2011
Polymers2,2011
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / 100closest to average structure
RepresentativeModel #1closest to the average

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Components

#1: Protein/peptide VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN /


Mass: 2200.629 Da / Num. of mol.: 1 / Fragment: L45 SEGMENT OF THE SHAKER POTASSIUM CHANNEL / Source method: isolated from a natural source / Source: (natural) Drosophila melanogaster (fruit fly) / References: UniProt: P08510

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111DQF-COSY
121TOCSY
131NOESY
NMR detailsText: This structure was determined using standard 2D homonuclear techniques.

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Sample preparation

DetailsContents: 2mM NA-L45 / Solvent system: TFE:H2O ratio of 80:20 with 9% D2O
Sample conditionsIonic strength: 0 / pH: 2.7 / Pressure: ambient / Temperature: 300 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Varian INOVAVarianINOVA6001
Varian INOVAVarianINOVA7502

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Processing

NMR software
NameVersionDeveloperClassification
DYANA1.5Guentert et al.structure solution
DYANA1.5Guentert et al.refinement
RefinementMethod: Hybrid distance geometry, simulated annealing / Software ordinal: 1
NMR representativeSelection criteria: closest to the average
NMR ensembleConformer selection criteria: closest to average structure / Conformers calculated total number: 100 / Conformers submitted total number: 1

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