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- PDB-1hno: CRYSTAL STRUCTURE OF PEROXISOMAL DELTA3-DELTA2-ENOYL-COA ISOMERAS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1hno | ||||||
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Title | CRYSTAL STRUCTURE OF PEROXISOMAL DELTA3-DELTA2-ENOYL-COA ISOMERASE FROM SACCHAROMYCES CEREVISIAE | ||||||
![]() | D3,D2-ENOYL COA ISOMERASE ECI1 | ||||||
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Function / homology | ![]() Beta-oxidation of very long chain fatty acids / Delta3-Delta2-enoyl-CoA isomerase / delta(3)-delta(2)-enoyl-CoA isomerase activity / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mursula, A.M. / van Aalten, D.M.F. / Hiltunen, J.K. / Wierenga, R.K. | ||||||
![]() | ![]() Title: The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase. Authors: Mursula, A.M. / van Aalten, D.M. / Hiltunen, J.K. / Wierenga, R.K. #1: ![]() Title: Crystallization and X-ray Diffraction Analysis of Peroxisomal Delta3-Delta2-enoyl-CoA Isomerase from Saccharomyces cerevisiae Authors: Mursula, A.M. / van Aalten, D.M. / Modis, Y. / Hiltunen, J.K. / Wierenga, R.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.2 KB | Display | ![]() |
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PDB format | ![]() | 49.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 31718.369 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Plasmid: PET3A / Production host: ![]() ![]() ![]() References: UniProt: Q05871, Delta3-Delta2-enoyl-CoA isomerase | ||
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#2: Chemical | ![]() #3: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.7 Å3/Da / Density % sol: 66.8 % | ||||||||||||||||||||
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: MES, ammonium sulfate, 1,4-dioxane, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 28, 1999 |
Radiation | Monochromator: synchrotron / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.5→20 Å / Num. all: 17313 / Num. obs: 17313 / % possible obs: 98.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.8 % / Biso Wilson estimate: 44.25 Å2 / Rmerge(I) obs: 0.066 / Net I/σ(I): 12.2 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.349 / Mean I/σ(I) obs: 4.9 / Num. unique all: 1669 / % possible all: 98.8 |
Reflection | *PLUS Lowest resolution: 50 Å |
Reflection shell | *PLUS Highest resolution: 2.5 Å / % possible obs: 98.8 % / Num. unique obs: 1669 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: enoyl-CoA isomerase liganded with perrhenate Resolution: 2.5→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 49.47 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.614 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 20 Å / σ(F): 0 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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