+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1hn3 | ||||||
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タイトル | SOLUTION STRUCTURE OF THE N-TERMINAL 37 AMINO ACIDS OF THE MOUSE ARF TUMOR SUPPRESSOR PROTEIN | ||||||
要素 | P19 ARF PROTEIN | ||||||
キーワード | ANTITUMOR PROTEIN / Arf / p19Arf / Tumor Suppressor (がん抑制遺伝子) / p53 (P53遺伝子) / mdm2 (Mdm2) | ||||||
機能・相同性 | 機能・相同性情報 : / senescence-associated heterochromatin focus / regulation of cell cycle => GO:0051726 / : / ubiquitin-protein transferase inhibitor activity / regulation of cell cycle => GO:0051726 / negative regulation of proteolysis involved in protein catabolic process / regulation of protein targeting to mitochondrion / negative regulation of mammary gland epithelial cell proliferation / 核小体 ...: / senescence-associated heterochromatin focus / regulation of cell cycle => GO:0051726 / : / ubiquitin-protein transferase inhibitor activity / regulation of cell cycle => GO:0051726 / negative regulation of proteolysis involved in protein catabolic process / regulation of protein targeting to mitochondrion / negative regulation of mammary gland epithelial cell proliferation / 核小体 / negative regulation of hepatocyte proliferation / regulation of apoptotic DNA fragmentation / positive regulation of DNA damage response, signal transduction by p53 class mediator / negative regulation of immature T cell proliferation in thymus / : / regulation of nucleocytoplasmic transport / somatic stem cell division / response to xenobiotic stimulus => GO:0009410 / : / autophagy of mitochondrion / positive regulation of protein sumoylation / mitochondrial depolarization / negative regulation of ubiquitin-protein transferase activity / positive regulation of apoptotic process involved in mammary gland involution / negative regulation of protein neddylation / negative regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of phosphorylation / cyclin-dependent protein serine/threonine kinase inhibitor activity / regulation of protein export from nucleus / regulation of DNA-binding transcription factor activity / SUMO transferase activity / negative regulation of ubiquitin protein ligase activity / negative regulation of cell-matrix adhesion / negative regulation of NF-kappaB transcription factor activity / negative regulation of B cell proliferation / regulation of cyclin-dependent protein serine/threonine kinase activity / somatic stem cell population maintenance / apoptotic mitochondrial changes / positive regulation of signal transduction by p53 class mediator / rRNA transcription / ubiquitin ligase inhibitor activity / protein K63-linked ubiquitination / transcription factor binding / negative regulation of cell cycle / NF-kappaB binding / ヘイフリック限界 / epidermis development / MDM2/MDM4 family protein binding / response to organonitrogen compound / regulation of G2/M transition of mitotic cell cycle / negative regulation of ubiquitin-dependent protein catabolic process / negative regulation of protein binding / negative regulation of protein kinase activity / protein destabilization / regulation of protein stability / negative regulation of cell growth / response to organic cyclic compound / G1/S transition of mitotic cell cycle / positive regulation of DNA-binding transcription factor activity / cellular response to hydrogen peroxide / positive regulation of protein localization to nucleus / protein polyubiquitination / rRNA processing / activation of cysteine-type endopeptidase activity involved in apoptotic process / positive regulation of cellular senescence / 細胞老化 / disordered domain specific binding / p53 binding / glucose homeostasis / 遺伝子発現の調節 / amyloid fibril formation / protein stabilization / positive regulation of apoptotic process / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / positive regulation of gene expression / 核小体 / protein kinase binding / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / protein-containing complex / ミトコンドリア / DNA binding / 核質 / 細胞核 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Mus musculus (ハツカネズミ) | ||||||
手法 | 溶液NMR / CNS was used for structure calculations | ||||||
データ登録者 | DiGiammarino, E.L. / Filippov, I. / Weber, J.D. / Bothner, B. / Kriwacki, R.W. | ||||||
引用 | ジャーナル: Biochemistry / 年: 2001 タイトル: Solution structure of the p53 regulatory domain of the p19Arf tumor suppressor protein. 著者: DiGiammarino, E.L. / Filippov, I. / Weber, J.D. / Bothner, B. / Kriwacki, R.W. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1hn3.cif.gz | 272 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1hn3.ent.gz | 226.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1hn3.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/hn/1hn3 ftp://data.pdbj.org/pub/pdb/validation_reports/hn/1hn3 | HTTPS FTP |
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-関連構造データ
類似構造データ |
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-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 4734.634 Da / 分子数: 1 / 断片: RESIDUES 1-37 / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / プラスミド: PET28A-POLYHIS-SYN-ARF N37 / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: Q64364 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: Backbone dihedral angle restraints were obtained using the program TALOS developed by Cornilescu, G., Delaglio, F. and Bax, A. |
-試料調製
詳細 | 内容: 1mM mArfN37 15N or 15N/13C; 10mM potassium phosphate, pH 5.0; 50mM NaCl 溶媒系: 5% D2O; 30% TFE (v/v) in water |
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試料状態 | イオン強度: 60 / pH: 5.0 / 圧: ambient / 温度: 313 K |
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Varian INOVA / 製造業者: Varian / モデル: INOVA / 磁場強度: 600 MHz |
-解析
NMR software |
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精密化 | 手法: CNS was used for structure calculations / ソフトェア番号: 1 | ||||||||||||||||||||||||
代表構造 | 選択基準: extended conformation | ||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 |