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Yorodumi- PDB-1hi9: Zn-dependent D-aminopeptidase DppA from Bacillus subtilis, a self... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1hi9 | ||||||
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Title | Zn-dependent D-aminopeptidase DppA from Bacillus subtilis, a self-compartmentalizing protease. | ||||||
Components | DIPEPTIDE TRANSPORT PROTEIN DPPA | ||||||
Keywords | HYDROLASE (PROTEASE) / PROTEASE / D-AMINOPEPTIDASE / DECAMER / SELF-COMPARTMENTALIZING | ||||||
Function / homology | Function and homology information Hydrolases; Acting on peptide bonds (peptidases); Aminopeptidases / sporulation resulting in formation of a cellular spore / aminopeptidase activity / metallopeptidase activity / metal ion binding Similarity search - Function | ||||||
Biological species | BACILLUS SUBTILIS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.4 Å | ||||||
Authors | Remaut, H. / Bompard-Gilles, C. / Goffin, C. / Frere, J.M. / Van Beeumen, J. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2001 Title: Structure of the Bacillus Subtilis D-Aminopeptidase Dppa Reveals a Novel Self-Compartmentalizing Protease Authors: Remaut, H. / Bompard-Gilles, C. / Goffin, C. / Frere, J.M. / Van Beeumen, J. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hi9.cif.gz | 266.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hi9.ent.gz | 219.2 KB | Display | PDB format |
PDBx/mmJSON format | 1hi9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hi/1hi9 ftp://data.pdbj.org/pub/pdb/validation_reports/hi/1hi9 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Details | THE ENZYME IS A HOMODECAMER WITH 52 POINT -GROUP SYMMETRY |
-Components
#1: Protein | Mass: 30276.670 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SUBTILIS (bacteria) / Strain: 168 / Description: RECOMBINANT EXPRESSION IN E. COLI / Cellular location: CYTOPLASM / Gene: DCIAA / Cellular location (production host): CYTOPLASM / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): B834(DE3) / References: UniProt: P26902 #2: Chemical | ChemComp-ZN / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43 % / Description: ANOMALOUS SCATERRERS: 2 ZN2+/274AA. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: 18% PEG6000, 100MM TRIS, PH 8.5, 5MM NACL, 5MM MGCL2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7B / Wavelength: 0.8445,1.2832,1.2834 | ||||||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 15, 1999 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.403→20 Å / Num. obs: 51831 / % possible obs: 96.9 % / Redundancy: 3.48 % / Biso Wilson estimate: 48.6 Å2 / Rsym value: 0.055 / Net I/σ(I): 11.7 | ||||||||||||
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 3.5 % / Mean I/σ(I) obs: 3.7 / Rsym value: 0.247 / % possible all: 98.6 | ||||||||||||
Reflection | *PLUS Num. measured all: 438502 / Rmerge(I) obs: 0.055 | ||||||||||||
Reflection shell | *PLUS % possible obs: 98.6 % / Rmerge(I) obs: 0.247 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.4→15 Å / Data cutoff high absF: 10000 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 Details: INITIAL REFINEMENT WITH REFMAC DURING MODEL BUILDING
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Displacement parameters | Biso mean: 44.9 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→15 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS |